黄芪甲苷预处理对脑缺血再灌注后外周型苯二氮(艹卓)受体表达的影响

摘要

Objective To invesligale the effects of Aslragaloside IV ( AST) on the expression of Peripheral Benzo-diazepine Receplors(PBRs)afler cerebral ischemia/reperfusion(IR) injury in rals. Methods All male SD rats were randomly divided into sham —operation, the IR and AST trealmenl groups ( 10 mg/kg, 40 mg/kg and 100 mg/kg). The rats in trealmenl group were trealed with AST injection intraperitoneally in 30 minutes before ischemia. Results The expression levels of PBRs increased in sham —operation group. Compared with IR group, the score decreased in AST 40 mg (2. 0 ±0. 4; ρ = 0. 002 ) and 100 mg groups ( 2. 2 ±0. 5 ;ρ = 0. 022 ) . The infarction volume decreased in AST 40 mg ( 72. 40 ±12. 31 ;ρ = 0.001) and 100 mg groups (75. 70 ±18. 34 ;ρ =0. 001) . The expression levels of PBRs and microglia expressing PBRs within penumbra diminished in 40 mg ( 51. 43 ±3. 01; ρ = 0. 001) and 100 mg groups ( 53. 65 ±2. 07 ; ρ = 0. 018). Compared with 10 mg group, the expression levels of PBRs and microglia expressing PBRs within penumbra diminished in 40 mg group (51. 43 ±3. 01 ;ρ = 0. 010) . Conclusions Aslragaloside IV prolecls neuronal cells againsl focal cerebral ischemia —reperfusion and its proleclion may be associated wilh its inhibitory action on PBRs.%目的 研究黄芪甲苷(astragaloside IV,AST)预处理对大鼠脑缺血再灌注损伤后外周型苯二氮(艹/卓)受体(peripheral benzodiazepine receptors,PBRs)表达的影响.方法将大鼠随机分为5组:假手术组,模型组,AST 10 mg组(10 mg/Kg体重),AST 40 mg组(40 mg/Kg体重),AST 100 mg组(100 mg/Kg体质量).各药物干预组分别于缺血前30 min经腹腔注射相应剂量的AST.结果模型组 PBRs表达明显增多.与模型组相比,AST 40 mg组神经功能障碍评分减少(2.0±0.4;P=0.002),AST 100 mg组神经功能障碍评分减少(2.2±0.5;P=0.022),AST 40 mg梗死体积减少(72.40±12.31;P=0.001),AST 100 mg梗死体积减少(75.70±18.34;P=0.001).AST 40 mg组半暗带区PBRs表达和表达PBRs的小胶质细胞数明显减少(51.43±3.01;P=0.001),AST 100 mg组半暗带区PBRs表达和表达PBRs的小胶质细胞数减少(53.65±2.07;P=0.018).与AST 10 mg组相比,AST 40 mg组半暗带区PBRs表达和表达PBRs的小胶质细胞数量减少(51.43±3.01;P=0.010).结论 AST对脑缺血再灌注后神经细胞具有明显的保护作用,其机制可能与抑制PBRs过度表达有关.