阿萨希毛孢子菌对人外周血单核源树突状细胞表型和功能的影响

摘要

Objective To investigate the effects of Trichosporon asahii on the phenotypic and functional maturation of human pe?ripheral blood monocyte?derived dendritic cells (DCs).Methods The monocyte?derived DCs of health donors were obtained by cul?ture in vitro.In the experimental groups,DCs stimulated by different doses of heat?inactived T.asahii conidia (DCs : T.asahii=1 : 1, 1 : 5,1 : 10) ,RPMI?1640 and lipopolysaccharide ( LPS) stimulation groups were as the blank control and positive control,respec?tively.Shapes of the cells were observed under the inverted microscope,the rate of phagocytosis was calculated by Wright Giemsa stai?ning.DCs maturation was analysed by using flow cytometry.Results The morphology of DCs was significantly changed during incuba?tion.At 24 h,more than one T.asahii conidias were efficiently bound and internalized by DCs and the phagocytic rates were signifi?cantly different between the experimental groups (P<0.05).The expressions of CD80,CD86,CD83 in the experimental groups were much higher than the blank control group (P<0.05),but significantly lower than LPS group (P<0.05).Conclusion T.asahii can change the morphology of DCs and promote the expression of surface molecules,which enhance the maturation of the dendritic cells.%目的 通过体外研究热灭活阿萨希毛孢子菌 (Trichosporon asahii,T.asahii)孢子对健康人外周血单核源树突状细胞(dendritic cells,DCs)的表型和功能的影响,探讨DCs在T.asahii感染中可能发挥的作用. 方法 体外诱导培养正常人外周血DCs,与不同浓度的热灭活T.asahii孢子进行孵育,DCs与T.asahii浓度比分别为1 : 1、1 : 5和1 : 10,RPMI?1640及脂多糖 ( lipopolysaccharide,LPS)刺激组分别作为空白对照及阳性对照,在显微镜下观察各组DCs形态的变化;瑞氏?姬姆萨染色观察实验组DCs对T.asahii的吞噬情况并计算吞噬率;流式细胞术检测各组DCs表面共刺激分子表达情况. 结果 诱导孵育过程中DCs形态发生明显改变;24 h时实验组部分DCs内可观察到不止一个被吞噬的T. asahii孢子,吞噬率组间差异有统计学意义 ( P<0.05);与空白对照组相比,实验组DCs形态发生明显改变,且表面共刺激分子CD80、CD86、CD83表达水平明显升高 ( P<0.05) ,但升高水平低于LPS刺激组,差异有统计学意义 ( P<0.05). 结论 人外周血DCs吞噬热灭活的T. asahii孢子后,形态发生改变,表面分子表达水平升高,DCs进一步成熟.