细胞骨架结构改变对软骨细胞表型的影响

摘要

Objective To investigate the effects of the changes of cytoskeleton on chondrocyte phenotype.Methods Rabbit articular chondrocytes were cultures in two-dimensional (2D) monolayer and three-dimensional (3D) pellet.We disrupted the tubulin,actin and vimentin of the cytoskeleton of chondrocyte for analyzing the relationship between cytoskeleton changes and chondrocyte phenotype.Results The vimentin disruption of chondrocyte didn't cause any significant changes in the expression of type Ⅰ collagen,but resulted in the decreased expression of type Ⅱ collagen and aggrecan,and the decrease was greater for chondrocytes in 3D pellet culture than that in 2D monolayer culture.The tubulin disruption of chondrocyte showed little effects on the expression of type Ⅰ collagen,type Ⅱ collagen and aggrecan.The actin disruption of chondrocyte promoted the expression of type Ⅱ collagen in chondrocyte in 2D monolayer culture not that in chondrocyte in 3D pellet culture.Conclusion Vimentin plays an important role in maintaining normal chondrocyte phenotype.%目的:分析细胞骨架结构改变对软骨细胞表型的影响.方法:二维及三维微团培养兔膝关节软骨细胞,分别破坏软骨细胞骨架的微管、肌动蛋白及波形蛋白,分析细胞骨架改变引起软骨细胞表型改变的关系.结果:破坏软骨细胞骨架的波形蛋白,可引起Ⅱ型胶原蛋白及蛋白聚糖表达下降,且三维微团培养较二维培养下降更明显,而Ⅰ型胶原蛋白表达则无明显改变:破坏软骨细胞骨架的微管,不引起Ⅰ型胶原蛋白、Ⅱ型胶原蛋白及蛋白聚糖表达的改变;破坏软骨细胞骨架的肌动蛋白,在二维培养条件下可促进Ⅱ型胶原蛋白的表达,而在三维微团培养条件下,Ⅱ型胶原蛋白表达则无明显改变.结论:波形蛋白对维持正常软骨细胞表型起重要作用.