SonoVue剂量与健康实验兔肾超声造影效果关系的实验研究

摘要

Purpose To study the effects of different doses of ultrasound contrast agent SonoVue upon contrast-enhanced ultrasound (CEUS) in the kidney of healthy rabbits, and to seek the optimal dose of SonoVue. Materials and Methods CEUS was performed in 10 healthy rabbits with GE LOGIQ-E9 by using 8 different doses of SonoVue (ranging from 0.02 ml/kg to 0.16 ml/kg). The quantitative parameters of the time-intensity curve (TIC) were measured and statistically compared. Results The TICs showed that the peak intensity (PI) and the area under curve (AUC) increased with dose when the doses ranged from 0.02 ml/kg to 0.10 ml/kg (r=0.962 and 0.965, P<0.05); when the dose further increased, AUC had little change but PI decreased reversely. The arrival time (AT) shortened along with the increase of the SonoVue dose (r= - 0.917, P<0.05). The dose had a positive correlation with time to peak (r=0.49, P<0.05). Conclusion The parameters of TIC are influenced intensely by different doses of SonoVue. It is important to realize the relationship between SonoVue doses and its effects upon contrast-enhancement ultrasound in microcirculatory quantification. The dose of 0.10 ml/kg appears to be the optimal dose for CEUS in examining kidney of healthy rabbit.%目的探讨不同 SonoVue 剂量对健康实验兔超声造影（CEUS）效果的影响，并分析其变化规律，探讨最佳 SonoVue 剂量。材料与方法采用 GE LOGIQ-E9超声诊断仪，分别对10例兔肾在8个 SonoVue 剂量水平（0.02~0.16 ml/kg）行 CEUS检查，通过时间-强度曲线测量肾血流灌注参数，并进行定量分析。结果 SonoVue在0.02~0.10 ml/kg 剂量范围内，峰值强度（PI）及曲线下面积（AUC）随剂量增加而增大，SonoVue 剂量与 PI、AUC 呈显著正相关（r=0.962、0.965，P<0.05）；进一步增加剂量，AUC 变化不明显，PI 反呈略下降趋势。到达时间（AT）随 SonoVue剂量的增加而呈逐渐缩短，两者呈显著负相关（r=－0.917，P<0.05）；SonoVue 剂量与达峰时间（TTP）呈正相关（r=0.49，P<0.05）。结论 SonoVue 剂量会影响CEUS 定量分析结果，确定剂量和效果的关系及认识其规律是进行 CEUS 和定量分析的前提条件。应用 SonoVue 对健康实验兔肾行 CEUS 定量分析时，0.10 ml/kg 是较理想的剂量。