不同时期卵巢癌移植瘤的携MMP-2抗体靶向超声造影剂诊断效能

摘要

Purpose To compare the characteristics of targeted ultrasound contrast agents of ovarian carcinoma xenograft tumor at different stages with SonoVue serving as the contract, and to explore the application value of targeted ultrasound contrast agents in diagnosis of ovarian carcinoma. Materials and Methods Fifty BALB/cNude nude mice were randomly divided into 5 groups with 10 in each group to establish subcutaneous xenograft tumor model of ovarian carcinoma. According to different inoculation time (14, 21, 28, 35, 42 d), 5 groups of nude mice underwent common contrast-enhanced ultrasound (MBc) and targeted ultrasound contrast (MBt) examination. The peak intensity (PI) and time to peak (TTP) of xenograft tumors were calculated by time-signal intensity curves, and their angiographic features were dynamically observed. Results PI in MBt group was higher than that in MBc group at different times, the difference of which was all statistically significant (P<0.05); TTP was shorter than that of MBc group, the difference between 14, 21 and 28 d was statistically significant (P<0.05); TIC curve was steeper at the early stage of inoculation. The difference between PI and TTP in MBt group was statistically significant (P<0.05). The difference of PI between 14 and 21 d, 21 and 28 d, 28 and 35 d were all statistically significant (P<0.01); differences of TTP between 14 and 21 d, 28 and 35 d were all statistically significant (P<0.01); the difference of PI and TTP compared within MBc group had no statistical significance (P>0.05). Conclusion In the early stage of inoculation, the contrast parameters PI and TTP carrying MMP-2 antibody-targeted ultrasound contrast agents are more advantageous than sonovue, which is helpful for the early diagnosis of ovarian carcinoma.%目的 对比不同时期卵巢癌移植瘤的携MMP-2抗体的靶向超声造影剂特征;以声诺维为参照,探讨其诊断卵巢癌的价值.材料与方法 选取50只BALB/cNude裸鼠并随机分为5组,每组10只,建立卵巢癌皮下移植瘤模型.根据不同接种时间(14、21、28、35、42 d)分别对5组裸鼠进行靶向超声造影(MBt)及普通超声造影剂(MBc)声诺维检查.应用时间-信号强度曲线计算移植瘤峰值强度(PI)、达峰时间(TTP),并动态观察其造影特征.结果 不同时期MBt组PI值均高于MBc组,差异均有统计学意义(P<0.05);TTP值均短于MBc组,14、21、28 d差异有统计学意义(P<0.05);时间-信号强度曲线在接种早期陡峭更明显.MBt组PI与TTP值组内比较,差异均有统计学意义(P<0.05).其中PI值在14 d与21 d、21 d与28 d、28 d与35 d比较,差异均有统计学意义(P<0.01);TTP值在14 d与21 d、28 d与35 d比较,差异有统计学意义(P<0.01);MBc组PI与TTP值组内比较,差异均无统计学意义(P>0.05).结论 接种早期,携MMP-2抗体靶向超声造影剂的造影参数PI、TTP较声诺维更具有优势,有助于卵巢癌的早期诊断.