NK 细胞及 IL-2、IL-4、IFN-γ在上呼吸道感染诱发急性分泌性中耳炎患者的表达

摘要

目的：检测上呼吸道感染诱发急性分泌性中耳炎患者急性期外周血 NK（CD3-CD16＋ CD56＋）细胞、B （CD3-CD19＋）细胞及 IL-2、IL-4、IFN-γ的变化。方法采用 BD CBA Flex Set IL-2、IL-4、γ-干扰素试剂盒，利用流式细胞术微球阵列法检测37例急性上呼吸道感染诱发急性分泌性中耳炎外周血3种细胞因子水平及 NK、B 淋巴细胞的比例。结果患者组 NK 细胞的百分比为（17．62±8．44）％，明显低于正常对照组，差异有统计学意义（P ＜0．05）；B 细胞在患者外周血中的比例为（13．44±5．41）％，比例明显高于正常对照组，差异有统计学意义（P ＜0．05）；患者组外周血 IL-2、IL-4、IFN-γ浓度与正常对照组比较无统计学意义（P ＞0．05）。结论急性分泌性中耳炎存在 NK 细胞低表达，B 淋巴细胞高表达，外周血细胞因子浓度无改变。%Objective This study aimed to investigate the change of NK,B lymphocyte and IL-2,IL-4,IFN-γ in se-cretory otitis media patients.The levels of three cytokines (IL-2,IL-4,γ-interferon)in the serum from 37 cases of acute upper respiratory tract infection induced secretory otitis media were detected by flow cytometry and BD CBA Flex Set kit.Peripheral blood samples of thirty-seven patients and ten healthy donors were prepared and then analyzed the per-centage of NK and B lymphocytes by tri-colour flow cytometry.The results were statistically analyzed.The percentage of NK in the patient group was significant lower than that in the control group(P <0.05).The percentage of B lympho-cytes was significant higher than that in the control group(P <0.05).The level of three cytokines in peripheral blood of SOM patients had no significant difference with the control group (P >0.05).Immune imbalance was exsisted with a-cute secretory otitis media patients.