MICB 0106等位基因与湖北汉族人溃疡性结肠炎的相关性

摘要

Objecfive To investigate the association between the exon 2,3,4 of MHC class Ⅰ chain-related gene-B(MICB)and ulcerative colitis(UC)in Chinese Han.Methods Using polymerase chain reaction single-stranded conformation polymorphism,allele frequency of MICB exon 2,3 and 4 in 105 patients with UC and 213 age and sex matched healthy controls were genotyped.All of the studied individuals were Chinese Han.Results Allele frequency of MICB 0106 was increased in patients with UC as compared with normal controls(19.0%vs 8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI:1.488-3.879).The frequency of MICB 0106 was increased significantly in patients with extensive colitis (24.4%vs 8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI:1.800-6.027),moderate and severe disease(24.1%vs 8.9%,P=0.000.Pc＜0.001,OR=3.294 95%CI:1.893-5.576)and in those with extra intestinal manifestations(20.5%vs 8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI:1.418-4.861).Furthermore,MICB 0106 allele was higher in frequency in the male patients with UC (22.1%vs 8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI:1.737-6.178)and the patients more than 40 years old(28.8%vs 8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI:2.381-8.504)as compared with healthy controls.Conclusion MICB 0106 allele is positively associated with UC,especially with extensive colitis,moderate and severe disease,presence of extra intestinal manifestations,male gender and age of more than 40 years in Chinese Han in Hubei province.%目的 MHC Ⅰ类相关基因(MHC class Ⅰ chain-related gene,MIC)与MHC Ⅰ类基因连锁,同源性很高.我们的前期研究发现MICA和MICB微卫星多态性与溃疡性结肠炎(UC)相关,本研究继续探讨MICB第2、3、4外显子基因多态性与UC的相关性,以期发现UC的易感基因.方法 采用聚合酶链反应-单链构象多态性方法(PCR-SSCP),对105例湖北汉族UC患者和213例正常对照者进行MICB基因分型.结果 UC患者的MICB 0106等位基因频率较正常组显著增高(19.0%比8.9%,P=0.000,Pc＜0.001,OR=2.402,95%CI为1.488～3.879).在临床亚型分析中,MICB 0106等位基因频率在全结肠炎、中重度UC组及有肠外表现的UC患者中均显著增高(分别为24.4%比8.9%,P=0.000,Pc＜0.001,OR=3.294,95%CI为1.800～6.027;24.1%比8.9%,P=0.000,Pc＜0.001,OR=3.249,95%CI为1.893～5.576;20.5%比8.9%,P=0.002,Pc=0.012,OR=2.626,95%CI为1.418～4.861).而且MICB 0106等位基因频率在男性和年龄≥40岁的患者中比正常组显著增高(分别为22.1%比8.0%,P=0.001,Pc=0.006,OR=3.276,95%CI为1.737～6.178;28.8%比8.3%,P=0.000,Pc＜0.001,OR=4.500,95%CI为2.381～8.504).结论 MICB 0106等位基因与湖北汉族人UC存在显著相关性,而且与全结肠炎、中重度UC、肠外表现、男性及年龄≥40岁的患者有关,提示携带MICB 0106等位基因的个体对UC存在遗传易感性.