纳米孔硅灰石载药抗菌止血材料的研究

摘要

A nanoprorous wollastonite (np-WT) was fabricated by using amphiphilic pluronic polymer surfactant (P123, EO20PO70EO20) as templates and sol-gel process, and a hemostatic agent of np-WT loaded drug (Vancomycin Hydrochloride) with antibacterial properties was investigated. The results showed that np-WT with large surface area and well ordered pores of around 2 nm could significantly shorten prothrombin time (PT) and the activated partial thromboplastin time (APTT). The np-WT loaded drug had no obvious effects on its hemostatic properties.The np-WT could slowly release drug into phosphate buffered saline(PBS) for 7 d. The np-WT loaded drug without cytotoxicity had good antibacterial properties. The np-WT loaded drug could shorten the bleeding time of rabbit back wound and control hemorrhage, showing good hemostatie properties.%采用溶胶-凝胶法,以聚氧乙烯-聚氧丙烯-聚氧乙烯三嵌段共聚物(R123)为模板剂,合成了纳米孔硅灰石(np-WT),用np-WT载盐酸万古霉素研制了一种新型的载药抗菌止血材料,并对其止血性能进行了研究.结果表明:np-WT具有排列有序的纳米孔道结构,其孔径在2 nm左右,高比表面积的np-WT能够明显地缩短体外部分凝血活酶时间(APTT)和凝血酶原时间(PT).载药np-WT能够在磷酸缓冲溶液(PBS)中缓慢地释放药物,载药np-WT对其凝血性能没有明显的影响.载药np-WT对大肠杆菌有很好的抗菌作用,细胞毒性实验表明:载药np-WT无细胞毒性.载药np-WT具有很好的止血性能,能够阻止兔背部伤口的流血和缩短其流血时间.