具有肿瘤荧光成像性能的核壳纳米过氧化氢酶模拟物

摘要

Microwave synthesis approach has been developed for preparing Mn-silica core-shell nano-complexes with fluorescent imaging by assembling the Mn(Ⅱ) complexes of bis(2-pyridylmethyl)amino-2-propionic acid (Adpa) on the surface of silica core-shell nanoparticles.IR,UV,TEM were used to characterize the structure of nanocomplexes.The results of H2O2 disproportionation show that Mn-silica core-shell nano-complexes have good analog characteristics of catalase as a new kind of nano-models for eatalase.Cell fluorescence image in vitro indicates that these Adpa modified nanoparticles locate outside of tumor cells,in contrast,Mn-silica core-shell nano-complexes could enter tumor cells which enables simultaneous tumor-targeting and good fluorescent imaging as a new tumor imaging agent.%运用微波法在硅核壳荧光材料的表面修饰了2-(二吡啶甲胺基)丙酸的锰配合物,获得具有荧光性能的锰-硅核壳纳米结构复合物,运用IR,UV,TEM等方法表征了纳米复合物的结构.H2O2岐化实验显示锰-硅核壳纳米复合物具有较好的过氧化氢酶模拟特性,是一种新的纳米过氧化氢酶模拟物.体外细胞荧光成像研究表明2-(二吡啶甲胺基)丙酸修饰的纳米球不能进入肿瘤细胞内,而锰-硅核壳纳米复合物能进入肿瘤细胞内,具备良好的肿瘤靶向性,显著提高肿瘤荧光成像效果,可作为新型的肿瘤成像剂.