电针对单纯性肥胖大鼠胰岛素抵抗及下丘脑刺鼠基因相关蛋白、神经肽Y的影响

摘要

目的：观察电针对饮食诱导的单纯性肥胖大鼠体质量、胰岛素抵抗及下丘脑刺鼠基因相关蛋白（AGRP）、神经肽Y（NPY）基因表达的影响，探讨电针治疗肥胖症的相关机制。方法50只SD雄性大鼠随机分为低脂组10只和高脂组40只，高脂组大鼠造模成功后，随机分为模型组、电针组、西药组，每组10只。电针组电针“后三里”和“曲池”，每日1次，每次20 min，连续28 d；西药组予奥利司他灌胃，低脂组和模型组不做治疗。每日记录大鼠体质量，检测空腹血糖和胰岛素，RT-qPCR检测下丘脑AGRP和NPY基因表达水平，HE染色观察脂肪组织和肝脏形态。结果治疗后电针组和西药组体质量、胰岛素抵抗指数、AGRP、NPY、脂肪细胞直径均低于模型组（P＜0.05），2组比较差异无统计学意义。电针组和西药组肝脏脂变情况均得到改善。结论电针通过改善胰岛素抵抗，抑制NPY和AGRP神经元的表达，达到控制体质量增长的目的。%Objective To observe the effects of electro-acupuncture (EA) on body weight, insulin resistance, hypothalamic agouti gene-related protein (AGRP) and neuropeptide Y (NPY) in diet induced obesity (DIO) rats; To discuss its mechanism for DIO.Methods Fifty SD male rats were randomly divided into low fat diet groups (LFD) and high fat diet group. After the DIO models were established, the successful model rats were randomly divided into model group, EA group and Orlistat (OLST) group. EA was applied to “Zusanli” (ST36) and “Quchi” (LI11) for 20 minutes. The treatment was done once a day for 28 days. OLST was treated with orlistat by gavage. LFD and model did not receive treatment. Body weight was recorded every day. FPG and FINS were detected. The expressions of AGRP and NPY were detected by RT-QPCR. Morphological changes of adipocyte and liver were examined by HE staining.Results The body weight, HOMA-IR, AGRP, NPY and diameter of adipocyte of EA group and OLST group were significantly lower than model group (P<0.05), difference between EA group and OLST group. The states of hepatic steatosis were improved in EA group and OLST group.Conclusion EA has the effects on weight loss by inhibiting the expressions of AGRP and NPY by improving IR.