江苏省三起甲型副伤寒爆发菌株与散发菌株的基因型及药物敏感性比较

摘要

Objective To study the genotypes and drug resistance of S.paratyphi A strains isolated from some sporadic cases and three outbreak cases in different areas of Jiangsu province.Methods Random amplified polyrnorphic DNA(RAPD)fingerprinting method was used to study the genotypes of 31 isolates.while the susceptibility of which to 12 antibiotics was determined by Kirby-Bauer method.Results 4 genotypes were found in 31 isolates.Genotype Ⅰ was dominant both in sporadic and outbreak isolates. The resistance rates of outbreak isolates to streptomycin,ampicillin and cephradine ranged from 4.6%～25.9%.Conclusions RAPD fingerprinting method can be used to distinguish the genetic diversity in the isolates with same serotype and the isolates with genotype Ⅰ seems to be dominant in the infections of Salmonella paratyphi A in our province.%目的 比较江苏省三地区连续发生的甲型副伤寒爆发与散发菌株在分子水平的联系,及其药物敏感性.方法 采用随机扩增多态性DNA(RAPD)分析方法,对爆发及散发菌株进行基因分型,采用K-B法对两类菌株进行药敏测定.结果 31株菌呈四个基因型图谱,爆发与散发菌株中基因Ⅰ型皆占优势,爆发株对链霉素、氨苄西林、头孢拉啶的耐药率从4.6%～25.9%.结论 RAPD方法能给予表型相同的甲型副伤寒菌株进行基因分型,基因Ⅰ型为江苏省甲型副伤寒的优势菌株.