HAART治疗1年对HIV/AIDS患者免疫重建效果观察

摘要

Objective To investigate the impact of one-year highly active antiretroviral therapy ( HAART) on antiviral effect and immune reconstruction in HIV/AIDS patients. Methods Thirty-five HIV/AIDS patients were initially treated with HAART when CD4 + T cells were <350/μL, blood samples were taken at the HAART time point 0,6,12 months, the virus load was tested by RT-PCR, CD4 + T cells, CDS + T cells, CD4 + naive cells, CD4 + memory cells and CDS + activated cells were detected by flow-cytometry. Results The average virus load of 35 patients was (4. 62 ± 1. 09) × 106 copies/mL before HAART, and was lower than the lower limit of detection value after HAART for 6 months and 12 months. The levels of CD4 + T cells, CD4 + naive cells and CD4 + memory cells were (312 ± 109. 22) /μL, (150 ± 57. 34)/μL and (212 ± 48. 06)/μL respectively after HAART for 12 months, while they were (183 ± 83. 73)/μL, (73 ± 20. 40)/μL and (119 ± 30. 42)/μL respectively at the baseline (P<0. 01), but they were still lower than healthy control group([768 ± 146. 41]/μL, [424 ± 87. 06]/μL and [442 ± 61. 40]/μL, respectively)(P<0. 05) ;the level of CDS + T cells were(427 ± 99. 79)/μL after HAART, which decreased significantly compared with (597 ±111. 43)/μL of the baseline (P<0. 05), but still higher than (208 ± 37. 39)/μL of healthy control group (P<0. 05). Conclusion HAART can both inhibit virus replication rapidly and reconstruct HIV/AIDS patients' immunity partially.%目的 研究高效抗反转录病毒治疗(HAART)1年,对HIV/AIDS患者免疫重建效果的影响.方法 确认人免疫缺陷病毒(HIV)感染者35例,检测CD4+T淋巴细胞<350/μL,开始HAART治疗,并于治疗0、6、12个月时分别检测其病毒载量及CD4+T淋巴细胞、CD8+T淋巴细胞、CD4+童贞细胞、CD4+记忆细胞、CD8+激活细胞计数.结果 治疗前,HIV RNA处于高复制水平,平均(4.62±1.09)×106copies/mL,HAART治疗6个月及12个月时均低于检测下限.患者的CD4+T淋巴细胞、CD4+童贞细胞和CD4+记忆细胞均明显增加,12个月时,各组细胞计数[分别为(312±109.22) /μL、(150±57.34)/μL、(212±48.06)/μL]与基线[分别为(183±83.73)/μL、(73±20.40)/μL、(119±30.42)/μL]比较,差异有统计学意义(P<0.01),但仍低于健康对照组 [分别为(768±146.41)/μL、(424±87.06)/μL、(442±61.40)/μL](P<0.05);CD8+T淋巴细胞计数(427±99.79)/μL较基线(597±111.43)/μL明显下降(P<0.05),但仍高于健康对照组(208±37.39)/μL(P<0.05).结论 HAART能快速抑制HIV复制,治疗1年能部分恢复HIV/AIDS患者免疫功能.