HLA-DRB1等位基因与吉林地区汉族乙肝疫苗免疫应答的关联研究

摘要

目的:探讨HLA-DRB1等位基因与吉林地区汉族乙肝疫苗免疫应答的关联性.方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对84例乙肝疫苗无或低应答者HLA-DRB1等位基因进行检测,与78例乙肝疫苗中或强应答者人群进行对照.结果:①HLA-DRB1*14等位基因频率在无或低应答组为23.8%,中或强应答组为5.13%,两组之间比较有统计学差异(P＜0.05);②HLA-DRB1*12、HLA-DRB1*15等位基因频率分别在无或低应答组为4.76%和7.14%,在中或强应答组为23.1%和24.4%,两组之间比较也存在统计学差异(P＜0.05).③等位基因HLA-DRB1*07(2.38%和5.12%),HLA-DRB1*08(9.52%和8.97%),HLA-DRB1*09(7.14%和10.3%),HLA-DRB1*11(7.14%和7.69%),HLA-DRB1*13(4.76%和6.41%)及HLA-DRB1*16(4.76%和5.13%),在无或低应答组和在中或强应答组之间基因频率分布没有统计学差异(P＞0.05).结论:吉林地区汉族乙肝疫苗接种后,①HLA-DRB1*14等位基因可能与无或低应答相关;②HLA-DRB1*12、15等位基因可能与中或强应答相关;③未检测到HLA-DRB1*07、08、09、11、13、16等位基因与免疫应答水平之间有明显的相关性.%Objective: To research the HLA-DRB1 alleles associated with immune response to hepatitis B vaccination in Chinese Han people of Jilin region.Methods: HLA-DRB1 alleles of 84 cases of non responders or hypo-responders to the Hepatitis B vaccination were detected by polymerase chain reaction sequence-specific primer (PCR-SSP), under the comparison with 78 cases of medium or hyper-responders.Results:①The allelic frequencies of HLA-DRB1 * 14 in non-responders or hypo-responders group was 23.8％, wheras in medium or hyper-responder group was 5.13 ％, shown significant differences between the two groups ( P ＜ 0.05); ②The allelic frequencies of HLA-DRB1 * 12 and HLA-DRB1 * 15 in non-responders or hypo-responders group was 4.76％ and 7.14％, wheras in medium or hyper-responders group was 23.1％ and 24.4％,it also showed significant differences between the two groups ( P ＜ 0.05) ;③The allelic frequencies of HLA-DRB1 *07 (2.38％ vs.5.12％ ) ,08(9.52％ vs.8.97％ ) ,09(7.14％ vs.10.3％ ), 11(7.14％ vs.7.69％ ), 13(4.76％ vs.6.41％), 16(4.76％ vs.5.13％ ) were no significant differences between the two groups ( P ＞ 0.05).Conclusion: Immune response to hepatitis B vaccine in Chinese Han people of Jilin region,①Non-response or hypo-responders might be related to HLA-DRB1 * 14 alleles;②HLA-DRB1 * 12,15 is associated with medium or strong responses;③No definite relations have been detected between HLA-DRB1 * 07,08,09, 11,13, 16 alleles and the level of immune response.