Nutlin-3促进肝癌细胞SMMC-7721发生焦亡的作用

摘要

目的:探讨MDM2拮抗剂Nutlin-3促进肝癌细胞SMMC-7721发生焦亡的作用及相关机制.方法:Western blot检测细胞中活化caspase-1(p20)及IL-1β的表达,LDH法检测SMMC-7721细胞焦亡情况,ELISA检测细胞上清中IL-1β释放情况.结果:Nutlin-3提高SMMC-7721细胞活化caspase-1(p20)及IL-1β的蛋白表达水平.Nutlin-3处理显著提高了 SMMC-7721 细胞培养上清中的LDH 及IL-1β的表达水平(P<0.05).结论:Nutlin-3激活了caspase-1,诱导肝癌细胞SMMC-7721发生焦亡,并促进IL-1β释放.%Objective:To evaluate the effect of Nutlin-3,a MDM2 antagonist,on the pyroptosis in SMMC-7721 cells.Methods: The expression of actived caspase-1(p20) and IL-1β was detected using Western blot analysis.Pyroptosis was investigated by a standard lactate dehydrogenase release assay(LDH).IL-1β content in the cell culture supernatant was quantified by ELISA.Results: Nutlin-3 up-regulated the expression level of actived caspase-1 and IL-1β in SMMC-7721 cells.Meanwhile,Nutlin-3 increased significantly the content of LDH and IL-1β in the cell culture supernatant(P<0.05).Conclusion: Nutlin-3 activated pro-caspase-1,promoted pyroptosis and IL-1β release in SMMC-7721 cells.