远志皂苷对APP/PS1双转基因小鼠海马神经细胞线粒体的保护作用

摘要

Objective To observe the regulation of the sengenin on APP/PSI double transgenic rats'hippocampus neuronal mito-chondria , explore the protection of the sengenin to the nerves .Methods APP/PSI double transgenic rats were divided into model , sengenin high, middle and low dose groups(18.5, 37.0 and 74.0 mg· kg-1· d-1), the same month old C57BL/6 rats were chosen as the control group.Hippocampus neuronal mitochondria potential was detected by immunofluorescence , cell apoptotic rate was measured by flow cytome-try, SOD, GSH-PX activity and MDA content were detected by colorimetry .Results Compared with control group , mitochondrial mem-brane potential was decreased , the apoptotic rate of neuron cell was increased , the activity of SOD ,CAT and GSH-PX were decreased signifi-cantly, the content of MDA of model group was increased significantly .Compared to model group , the mitochondrial membrane potential was increased, the apoptotic rate of neuron cell was decreased , the activity of SOD, CAT and GSH-PX were increased, the content of MDA was decreased significantly in each sengenin group (all P<0.05).Conclusions Sengenin could protect the function of hippocampal neurons by regulating mitochondria function .%目的 观察远志皂苷对APP/PS1双转基因小鼠海马神经细胞线粒体功能的调控,探讨远志皂苷的神经保护作用机制.方法 将APP/PS1双转基因小鼠分为模型组,远志皂苷低、中和高剂量组(18.5、37.0和74.0 mg·kg-1·d-1),选同月龄C57BL/6小鼠为空白对照组,采用免疫荧光检测海马神经细胞线粒体膜电位,流式细胞术检测细胞凋亡率,比色法等检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活力和丙二醛(MDA)含量.结果 与空白对照组相比,模型组线粒体膜电位降低,神经细胞凋亡率增高,SOD,过氧化氢酶(CAT)和(GSH-PX)活性明显降低,MDA含量明显增高;与模型组相比,远志皂苷各剂量组的线粒体膜电位升高,神经细胞凋亡率下降,SOD,CAT和GSH-PX活性明显升高,MDA含量明显降低(P<0.05).结论 远志皂苷可能通过调控线粒体功能,发挥保护海马神经元的作用.