Toll样受体2基因敲除对高脂饮食诱导的肥胖小鼠脂肪组织凋亡的影响

摘要

目的 分析Toll样受体(TLR)2敲除对高脂饮食诱导的肥胖小鼠脂肪组织凋亡的影响.方法 C57BL/6J小鼠和TLR2基因敲除小鼠各16只,分为两组,给予普通饮食和高脂饮食喂养:正常对照组(NC)、肥胖组(OB)、TLR2基因敲除组(TK)和TLR2基因敲除肥胖组(TO).16 w后检测各组空腹血糖(FPG),三酰甘油(TG),总胆固醇(TC),低密度脂蛋白(LDL)和高密度脂蛋白(HDL)水平及脂肪组织caspase3活性,bcl2和bax蛋白、mRNA表达量.结果 与NC组相比,OB组小鼠脂肪组织caspase3活性升高,bax蛋白和mRNA水平升高,bcl2蛋白和mRNA水平降低;与OB组相比,TO组小鼠脂肪组织caspase3活性降低,bax蛋白和mRNA水平降低,bcl2蛋白和mRNA水平升高.结论 TLR2敲除减轻了高脂饮食诱导的肥胖小鼠脂肪组织的细胞凋亡.%Objective To investigate the effect of TLR2 knockout upon the apoptosis in adipose tissue of high-fat-diet induced obe-sity mice.Methods 16 C57bl/6J mice and 16 TLR2-/-mice were divided into 4 groups with common chow or high-fat-diet:normal control, obesity,TLR2 gene knockout group and TLR2 gene knockout obesity groups. Fasting plasma glucose(FPG),triglyceride(TG),total cho-lesterol(TC),high density lipoprotein (HDL),and low density lipoprotein (LDL) in plasma as well as caspase3 activity in adipose tissue were measured. Expressions of bax and bcl2 were measured by Western blot and SYBR fluorescence RT-PCR.Results Compared with con-trol group,a higher caspase3 activity in adipose tissue was detected in obesity group,the levels of bcl2 and bcl2 mRNA were decreased while bax and bax mRNA were increased. Further more,compared with obesity group,there were lower caspase3 activity,bax and bax mRNA as well as higher bcl2 and bcl2 mRNA in TLR2 gene knockout obesity group. Conclusions Apoptosis is alleviated in adipose tissue of high-fat-diet induced obesity mice by TLR2 gene knockout.