大鼠感染后肠功能紊乱树突状细胞及血管活性肠态受体的变化

摘要

目的 观察福氏志贺氏痢疾杆菌感染后肠功能紊乱大鼠,肠道黏膜树突状细胞(DC)表面分子CD11c与共刺激分子CD80 、CD86及血管活性肠态受体(VIPR)1、2表达及意义.方法 将60只雄性Wistar大鼠随机分为对照组和实验组,用福氏志贺氏痢疾杆菌灌胃造感染后肠功能紊乱模型.行大鼠回肠末端和远端结肠大体形态和组织学评分,并通过免疫组化检测肠道黏膜表面分子CD11c、CD80、CD86及VIPR1和VIPR2的表达.结果 实验组大鼠与对照组相比,回肠末端和远端结肠的大体形态及组织学评分均无显著性差异(P＞0.05)；回肠末端CD11c、CD80及CD86阳性表达面积亦均无显著性差异(P＞0.05).而实验组大鼠远端结肠CD11c、CD80、CD86、VIPR2,以及回肠末端VIPR1、VIPR2阳性表达面积均显著高于对照组(P＜0.05).结论 感染后肠功能紊乱大鼠,DC细胞可能参与远端结肠免疫激活,VIPR2对DC细胞免疫具有调节功能；回肠末端VIPR1、VIPR2在肠功能紊乱发病过程中起重要作用.%Objective To investigate the expression of dendritic cells (DC) surface antigen CD11c, costimulatory molecules (CD80, CD86) and vasoactive intestinal polypeptide receptors (VIPR) in rats intestinal mucosa with Shigella flexneri infection. Methods Sixty male Wistar rats were divided into control group (n =30) and experimental group (n=30). The gross morphology and histology scores of terminal ileum and distal colon of the rats were evaluated in the experimental group with intestinal infection following intragastric administration of Shigella flexneri and the control group without intestinal infection. Immunohistochemistry revealed the expression of intestinal surface molecules CDllc, costimulatory molecules (CD80, CD86) and VIPR1 and VIPR2. Results There were no differences for gross morphology and histology scores of terminal ileum and distal colon and the immunohistochemistry-positive expression area of CDC11c, CD80 and CD86 between the control group and the experimental group (P >0.05). The experimental group had significantly higher immunohistochemistry-positive expression area of CDC11c, CD80, CD86 and VIPR2 from distal colon and that of VIPR1 and VIPR2 from terminal ileum compared with control group ( P < 0. 05 ). Conclusion DC cells possibly contributed to immune activation of the distal colon of intestinal infection rats while VIPR2 regulated the process of the immune activation. VIPR1 and VIPR2 may play important roles during the pathogenesis of the condition at terminal ileum of rats with intestinal infection.