幽门螺杆菌感染诱导胃上皮细胞miR-155的表达及意义

摘要

Objective To investigate the miR-155 expression in gastric mucosa of Helicobacter pylori (H.pylori) infected and its influence on the biological behavior of gastric epithelial cells.Methods Twenty-four gastric mucosal tissues of patients with chronic gastritis and H.pylori infected gastric epithelium cells (GES-1) in vitro were collected,the expression of miR-155 was detected by Real-time PCR in gastric mucosa and gastric epithelial cell line.The influence of miR-155 overexpression in gastric epithelial cells on cell proliferation and migration and autophagy was detected by CCK-8,scratch test and Western blotting.Results The level expression of miR-155 was higher in gastric mucosa of H.pylori infected than that in the negative group,the expression of miR-155 in GES-1 cells was stimulated by H.pylori.The results of CCK-8,scratch test established that miR-155 could promote the ability of proliferation and migration of gastric epithelial cells.Western blotting result showed that miR-155 also promoted the expression of autophagy protein LC3-Ⅱ.Conclusion H.pylori infection can promote the expression of miR-155 in gastric epithelial cells and affect its biological function and regulate the mucosal barrier function.%目的 探讨miR-155在幽门螺杆菌(Helicobacter pylori,H.pylori)感染性胃炎患儿胃黏膜组织的表达意义及对胃上皮细胞生物学功能的影响.方法 收集24例慢性胃炎患儿的胃黏膜组织及体外H.pylori感染胃上皮细胞株(GES-1),采用Real-time PCR方法检测胃黏膜组织和胃上皮细胞株miR-155的表达.通过转染的方法使胃上皮细胞miR-155高表达,采用CCK-8法、划痕实验、Western blotting检测miR-155对胃上皮细胞增殖、迁移功能及自噬蛋白表达的影响.结果 Real-time PCR结果显示,H.pylori感染阳性的胃黏膜组织miR-155表达水平高于非感染组,体外H.pylori感染诱导胃上皮细胞miR-155高表达;CCK-8、划痕实验结果显示,miR-155过表达可促进胃上皮细胞增殖和迁移能力;Western blotting结果显示,miR-155促进自噬蛋白LC3-Ⅱ的表达.结论 体内外实验证实,H.pylori感染促进胃黏膜表达miR-155,并影响胃上皮细胞的生物学作用,调控黏膜屏障功能.