花糕红点鲑多态性微卫星筛选及鸭绿江种群遗传多样性分析

摘要

The polymorphic primers for Dolly Varden Salvelinus malma as a rare and endangered cold-water species in China was screened from char S.confluentus by microsatellite,and the genetic diversity of the Yalu River population was analyzed.The results showed that 5 microsatellite primers screened from 10 microsatellite primers in Salvelinus malma were polymorphous in Dolly Varden.There were the number of alleles(Na) of 6~22(average 15),heteroerozygosity(H) of 0.4063~0.9333(average 0.7851) and polymorphic information content(PIC) of 0.716 4~0.919 8(average 0.8536) in Yalu River population of Dolly Varden.The findings indicated that the 5 primers from S.confluentus provided a good versatility in dolly varden,and a high level of genetic diversity in Yalu River population,which provides a genetics theoretical basis for the protection and management of germoplasm resources of Dolly Varden in China.%利用强壮红点鲑（Salvelinus confluentus）的微卫星筛选出适用于我国的珍稀濒危冷水性鱼类——花糕红点鲑（Salvelinus malma）的多态性引物,并分析其鸭绿江种群的遗传多样性。结果显示,在强壮红点鲑的10个微卫星引物中有5个在花糕红点鲑群体中具有多态性,鸭绿江种群的等位基因数（N）a为6~22（平均15）,杂合度（H）为0.4063~0.9333（平均0.7851）,多态信息含量（PIC）为0.7164~0.9198（平均0.8536）。强壮红点鲑微卫星引物在花糕红点鲑中具有很好的通用性,花糕红点鲑的鸭绿江种群具有较高的遗传多样性水平。本研究结果为我国花糕红点鲑种质资源保护和管理提供遗传学理论依据。