低温胁迫对马来西亚红罗非鱼血清生化指标的影响

摘要

The lethal temperature was studied in Malaysian red tilapia (Oreochromis mossambicusíO. niloticus) under indoor natural cooling, and serum biochemical indexes were tested at water temperature of 17.8℃ and beginning of death in the red tilapia. The re-sults showed that red tilapia death appeared when the temperature dropped to 8.6℃, with range from 8.6℃ to 6.4℃, and the LC50 low temperature of 7.6℃. There were significantly higher activities of ALT, AST, LDH, ALP and CK-MB at 8.6℃compared with at 17.8℃(P<0.05). The CREA level and the content of K+were found to be significantly increased (P<0.05) at 8.6℃, while TP, ALB, GLO and the content of Ca2+increased slightly. However, the contents of Na+and Cl-were significantly decreased (P<0.05), with slightly lower CHOL and TG levels.%在室内自然降温条件下，研究了马来西亚红罗非鱼(Oreochromis mossambicus×O. niloticus)致死低温，检测了在温室越冬温度（17.8℃）和低温胁迫下开始死亡时红罗非鱼血清的生化指标。结果表明，当水温降到8.6℃时，马来西亚红罗非鱼开始死亡，死亡低温范围为8.6~6.4℃，半致死低温为7.6℃；8.6℃时，红罗非鱼血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)和肌酸激酶同工酶(CK-MB)5种酶的活性显著高于17.8℃时(P<0.05)，肌酐(CREA)含量、K+浓度显著上升(P<0.05)，总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)，及Ca2+含量小幅增加，而Na+和Cl-含量均显著下降(P<0.05)，胆固醇(CHOL)和甘油三酯(TG)含量略有降低。