光合细菌球形红细菌降解HMX

摘要

The effects of oxygen supply and illumination,initial concentration of HMX,inoculation quantity,pH value and temperature on the degradation ability of HMX by domesticated Rhodobacter sphaeroides H strain were studied.Its kinetic equation of degradation was fitted and analyzed.The optimum conditions for the degradation of HMX by Rhodobacter sphaeroides were determined by changing one influencing factor and fixing the other four conditions.The concentration of HMX and the cell amount of Rhodobacter sphaeroides were measured by a spectrophotometer.The results show that domesticated Rhodobacter sphaeroides can efficiently degrade HMX,Rhodobacter sphaeroides H strain can grow commendably and degrade HMX under different conditions of oxygen supply and illumination,and the degradation rate reaches more than 70％.The best degradation condition is anaerobic illumination.The optimum conditions for the degradation of HMX are initial concentration of 100 mg · L-1,pH =7,inoculation quantity of 15％ and temperature of 30 ℃,the degradation rate of HMX is the highest,reaching 88.9％.The degradation process of HMX by Rhodobacter sphaeroides is consistent with the first order kinetic equation.%研究了供氧光照、奥克托今(HMX)初始浓度、接种量、pH值和温度对驯化球形红细菌(Rhodobacter sphaeroides)H菌株降解HMX能力的影响,对其降解动力学方程进行了拟合分析.通过改变1个影响因素,固定其它4个条件,确定了球形红细菌降解HMX的最适宜条件.用分光光度计对HMX浓度和球形红细菌细胞生物量进行了测定.结果表明,驯化后的球形红细菌可高效降解奥克托今,球形红细菌H菌株在不同供氧光照条件下均能很好生长并降解HMX,且降解率均达到70％以上,最佳降解条件为光照厌氧.该菌株降解HMX的最适条件为:HMX初始浓度为100 mg·L-1、pH值为7.0、接种量为15％,温度为30 c℃,在96 h降解率达到最高,为88.9％.球形红细菌降解HMX的过程符合一级反应动力学方程.