糖化血清蛋白对肾小球系膜细胞增殖和分泌Ⅳ型胶原的影响

摘要

ObjectiveTo study the relationship between glycated serum proteins and development of diabeticnephropathy by investigating effects of glycated serum proteins on mesangial cells.MethodsNormal human sera wasnonenzymatically glycated by incubation for 7 days with 28mmol/L glucose.The amount of 3H-Thymidine incorporationinto mesangial cells and secretion of type Ⅳ collagen in media were respectively measured when rat mesangial ceils hadbeen cultured with different concentrations of glycated serum proteins for 48 hours.Results Supplementation ofmesangial cells cultured in the presence of 3% and 10% glycated serum protein inhibited 3H-Thymidine incorporation by19% and 66% respectively comparing with the same concentrations of normal serum, which was dose-dependent onglycated serum proteins, and increased content of type Ⅳ collagen by 24% and 35.6% respectively.ConclusionTheglycated serum proteins played an important role in development of diabetic nephropathy by causing the expansion ofextracellular matrix in mesangial membrane area and inducing glomerulosclerosis.%目的研究糖化血清蛋白对培养的肾小球系膜细胞生长和分泌Ⅳ型胶原的影响，以了解糖化蛋白与糖尿病肾病的关系。方法正常人血清在28mmol／L葡萄糖的磷酸缓冲液中25 C孵育7天，使血清蛋白糖化，分别测定不同浓rn度的糖化血清蛋白对肾小球系膜细胞rn rnrn掺入和培养上清液中Ⅳ型胶原含量的影响。结果糖化血清rn蛋白浓度为3％和10％，可明显抑制３H-Thymidine掺人量且呈剂量依赖性。与相同剂量的正常血清比，分别抑制19％和66％，而Ⅳ型胶原分泌量分别增加24％和35.6％。结论糖化血清蛋白可能通过减少肾小球系膜细胞形成和增加系膜基质扩张而促进糖尿病肾脏的肾小球硬化。