目的 探讨高脂诱导IR apoE-/-小鼠胆固醇代谢基因的变化.方法 健康雄性apoE-/-小鼠,随机分为普食组(NF组)和高脂喂养组(HF组),共喂养16周.采用实时荧光定量PCR测定肝组织羟甲基戊二酸单酰辅酶A还原酶(HMGCR)、固醇调节元件结合蛋白-2(SREBP-2)、低密度脂蛋白受体(LDLr)基因mRNA表达.结果 HF组小鼠FBG、Fins、TC、TG、HDL-C、LDL-C和FFA水平均明显升高(P均<0.01).HF组小鼠肝脏HWGCR、SREBP-2和LDLr mRNA表达水平均明显降低(P<0.01或P<0.05).结论 在高脂喂养ap0E-/-小鼠,HMCA2R、SREBP-2和LDLr的变化在其胆固醇代谢紊乱中可能具有重要作用.%Objective To investigate the effects of high-fat induced insulin resistance (IR) on gene-related cholesterol metabolism in apoE-/- mice. Methods Healthy male apoE-/- mice were randomized into normal diet group (NF group, n=10) and high-fat group (HF group, n=10) and were fed for 16 weeks. The mRNA expressions of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), sterol regulatory element binding protein-2 (SREBP-2), and low density lipoprotein receptor (LDLr) of the hepatic tissues were measured by quantitative real-time PCR. Results Compared with the NF group, the fasting blood glucose (FBG), fasting plasma insulin (Fins), total cholesterol (TO, triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and free fatty acid (FFA) were significantly increased (all P<0. 01), while the mRNA expression of HMGCR, SREBP-2, and LDLr were significantly decreased in the hepatic tissues of apoE7 mice (P<0. 01 and P<0. 05) in the HF group. Conclusion The down-regulation of HMGCR, SREBP-2 and LDLr might contribute to the disorder of cholesterol metabolism in high-fat fed apoE-/- mice.
展开▼
