Objective To investigate the association between ApoC3 gene promoter region insulin response element -482C→T polymorphism and diabetic nephropathy. Methods The polymorphism of ApoC3 gene promoter region -482C→ T was detected by PCR-RFLP method in 179 unrelated Chinese with type 2 diabetes(DM group) and 60 normal controls (NC group). The genotype frequency, allele frequency and relative clinical data were compared between groups. Ressults The frequencies of T/T genotype and T allele were significantly higher in DN (DN1 + DN2) group than in DNO group(P<0. 01). However , there were no significant differences in genotype between DN1 and DN2 groups(P>0. 05). In DM (DNO + DN1 + DN2) group, triglyceride(TG) and 24h urinary albuminuria (Ualb) were significantly higher in T/T genotype group than in C/T and C/C genotype groups (P<0. 01). Logistic regression analysis showed that ApoC3 gene promoter region-482C→T polymorphism and diabetic duration were risk factors for DN occurrence. Conclusion The ApoC3 promoter region -482T/T genotype may be a risk factor of DN occurrence. Diabetic duration >5 years may be a risk factor for the progress of diabetic nephropathy in Chinese.%目的 探讨载脂蛋白C3(ApoC3)基因启动子区-482C→T多态性与中国人DN的相关性.方法 运用聚合酶链反应-限制性片段长度多态性技术(PCR-RFLP),在中国人群中对179例T2DM患者和60名正常对照者(NC)的ApoC3基因启动子区-482C→T的多态性进行检测,并比较各组问基因型频率和等位基因频率以及相关临床资料.结果 DN组(DNI+DN2)的T/T基因型频率及T等位基因频率高于DNO组(P<0.01),但DN2与ND1组间T/T基因型频率及T等位基因频率差异无统计学意义(P>0.05).T2DM组中,T/T基因型组患者的血TG水平及24h UAlb均高于C/T和c/c基因型组者(P<0.01).Logistic回归分析表明:ApoC3基因启动子区-482C→T多态性和DM病程与DN显著相关,-482T/T基因型是DN发生的危险因素DN患者中,DM病程>5年是DN2发生的危险因素.结论 在中国南方汉族人群中,ApoC3基因启动子区-482T/T基因型可能是DN发生的危险因素;病程>5年可能是DN进展的危险因素.
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