不同浓度肿瘤坏死因子对人牙周膜细胞增殖和碱性磷酸酶活性的影响

摘要

AIM:To study the effect of recombinant human tumor necrosis factor-a (TNF-a) on prolifera-tion and activity of alkaline phosphatase (ALP) of human periodontal ligament cells ( HPDLCs) cultivated in vitro. METHODS: The HPDLCs were tested Vimentin positive and Ck (pan) negative. Different dosages (Ong/Ml, lng/Ml, 5ng/Ml, lOng/Ml, 20ng/Ml) of TNF-a were added in the cultured HPDLCs. The proliferation of HPDLCs was examined by MTT assay during a period of 7 d. The Mrna expression of CyclinDl was tested by real time PCR after 72 h. ALP activity was examined under the same condition. RESULTS: Compared with the control group, 1 ng/Ml, 5 ng/Ml of TNF-a significantly elevated the proliferation rate and ALP activity of HPDLCs (P < 0. 05). However 10 ng/Ml, 20 ng/Ml of TNF-a significantly reduced the proliferation rate and ALP activity (P <0. 05). CONCLUSION: TNF-amay participate in the remodeling of periodontal tissue by regulating the proliferation and os-teogenic differentiation of HPDLCs.%目的:观察不同浓度肿瘤坏死因子(TNF-α)对人牙周膜细胞增殖活性和碱性磷酸酶(ALP)活性的影响.方法:取第4代体外培养人牙周膜细胞(hPDLCs),分别与0、1、5、10、20 ng/mL不同浓度TNF-α共同培养7d,采用MTT法测定培养后0～7d各时间点HPDLCs的增殖活力；用实时PCR检测不同浓度TNF -α刺激72 h后细胞周期蛋白(CyclinD1) mRNA的表达；检测ALP活性.结果:与对照组相比,1、5 ng/mL的TNF-α均可促进HPDLCs的增殖和Cyclin mRNA和ALP的表达(P＜0.05)；10、20 ng/mL浓度可抑制PDLCs的增殖和Cyclin D1 mRNA、ALP的表达(P＜0.05).结论:不同浓度的TNF-α刺激牙周膜细胞后可以影响细胞的增殖和分化.