手足口病患儿氧化应激指标及外周血淋巴细胞百分比检测结果分析

摘要

Objective To explore the clinical significance of serum malondialdehyde (MDA), super-oxide dismutase (SOD), oxidized low density lipoprotein (ox-LDL) levels and peripheral blood lymphocyte percentage changes in patients with hand-foot-and-mouth disease (HFMD). Methods 122 cases common (common HFMD group) and 96 cases severe (severe HFMD group) HFMD children and 100 cases healthy children in our hospital from March 2014 to June 2015 were collected. Serum MDA, SOD, ox-LDL levels and peripheral blood lymphocyte percentage were measured, and the results were analyzed statistically. Results There were statistical significance in the differences of MDA, SOD and ox-LDL levels among control group, common and severe HFMD group (Pall<0.05). The MDA and ox-LDL levels showed increasing trend, and SOD showed decrease trend along with pathogenetic condition development. There were statistical significance in the differences of MDA, SOD and ox-LDL levels between each two groups(Pall<0.05). There were statisti-cal significance in the differences of Ts cell, Th cell and NK cell percentage among control group, common and severe HFMD group (Pall<0.01), except B cell. The Ts cell, Th cell and NK cell percentage showed decrease trend along with pathogenetic condition development. There were statistical significance in the differences of Ts cell, Th cell and NK cell percentage between each two groups (Pall<0.05). The correlation coefficient be-tween peripheral blood lymphocyte percentages and serum levels of MDA, SOD, ox-LDL were all higher than 0.700 (Pall<0.05). Conclusion Serum MDA, SOD, ox-LDL levels and peripheral blood lymphocyte per-centage are closely correlated with the progress of HFMD. Serum MDA , SOD and ox-LDL content have a cer-tain correlation with the percentages of peripheral Th cell, Ts cell and NK cells.%目的 探讨手足口病(hand-foot-and-mouth disease, HFMD)患儿血清氧化应激指标水平变化及外周血淋巴细胞免疫分型检测的临床意义. 方法 选择2014年3月至2015年6月于我院就诊的218例HFMD患儿,其中普通HFMD患儿122例,重症HFMD患儿96例,同期选择我院100例健康体检儿童为健康对照组,检测受试儿童血清丙二醛(malondialdehyde, MDA)、超氧化物歧化酶(superox-ide dismutase, SOD)、氧化型低密度脂蛋白(oxidized low density lipoprotein, ox-LDL)含量及外周血淋巴细胞免疫分型,对检测结果进行统计学分析. 结果 健康对照组、普通HFMD组、重症HFMD组患儿血清MDA、SOD、ox-LDL的检测结果差异均有统计学意义 (P均<0.05). 随着病情的加重,MDA、ox-LDL水平呈升高趋势,SOD水平呈下降趋势,且各指标各组间两两比较差异均有统计学意义(P均<0.05).健康对照组、普通HFMD组、重症HFMD组外周血Th细胞、Ts细胞、NK细胞百分比差异均有统计学意义(P均<0.01),而B细胞百分比各组间差异无统计学意义(P>0.05). 随着病情的加重,外周血Th细胞、Ts细胞、NK细胞百分比呈现下降趋势,且各指标组间两两比较差异均有统计学意义(P均<0.05).HFMD 患儿血清中MDA、SOD 及ox-LDL 的含量与外周血各淋巴细胞百分比均呈明显的正相关性(r均>0.700,P均<0.05). 结论 HFMD患儿血清中MDA、SOD、ox-LDL的含量及外周血淋巴细胞百分比与HFMD病情的严重程度相关; 患儿血清中MDA、SOD、ox-LDL的含量和外周血Th、Ts、NK细胞百比均有相关性.