细胞因子诱导的杀伤细胞逆转耐顺铂肺腺癌细胞系A549/DDP 耐药性的研究

摘要

Objective: To observe the effects of cytokine-induced killer cells (CKs) on the reversal of cisplatin (DDP) resistance in the DDP-resistant human lung adenocarcinoma cell line A549/DDP. Methods: The cisplatin resistance of the A549/DDP cell line was validated and the drug sensitivity of A549/DDP indirectly cocultured with CIKs was determined with an MTT assay. The expression levels of the relative resistance genes of A549, A549/DDP, and A549/DDP co-cultured with CIK were determined via semiquantita-tive RT-PCR. The expression levels of relative resistance proteins were determined via western blot analysis. Results: The resistant index of A549/DDP to A549 was 14.5. The GST-π expression levels were increased in A549/DDP than in A549, but hCTR1 was contrary (P < 0.05 ). The cisplatin resistance of A549/DDP cocultured with CIK was decreased, and its GST-π expression was decreased (P < 0.05 ). Conclusion: CIK could reverse the cisplatin resistance of A549/DDP by inhibiting the expression of GST-π.%目的:观察细胞因子诱导的杀伤细胞(cytokine induced killer,CIK)对耐顺铂(DDP)人肺腺癌细胞系A549/DDP的耐药逆转作用及其逆转A549/DDP耐药的可能机制.方法:A549/DDP与CIK细胞采用Transwell非接触共培养.四氮甲唑兰比色法(MTT法)验证A549/DDP的DDP耐药性及检测共培养前后A549/DDP对DDP耐药性的变化.RT-PCR法筛选A549与A549/DDP有差异表达的基因作为检测耐药性变化的观察指标,并检测共培养前后A549/DDP中基因表达水平的变化;Western blot检测A549及共培养前后A549/DDP中基因蛋白水平的变化.结果:A549/DDP的耐药系数为14.5,具有较强的DDP耐药性.RT-PCR筛选出A549与A549/DDP表达有差异的耐药相关基因为谷胱甘肽转移酶(glutathione-S-transferase,GST-π)基因、人类铜离子转运蛋白(human copper transporter 1,hCTR1)基因,A549/DDP中GST-π表达量明显增加,hCTR1表达量明显降低.与CIK细胞共培养后,A549/DDP对DDP的耐药性明显下降,共培养20h后耐药逆转倍数约为4.93倍,细胞内GST-π基因及蛋白水平的表达明显降低(P<0.05).结论:CIK细胞对A549/DDP有逆转DDP耐药的作用,其机制可能与下调GST-π基因及蛋白水平的表达有关.