组蛋白H2B 单泛素化在原发性结肠腺癌中的表达及意义

摘要

Purpose To explore the role of histone H2B monoubiquitination in primary colonic carcinoma and clinicopathological pa-rameters of colonic carcinoma. Methods To detecte the expression of histone H2B monoubiquitination in 116 cases of primary colonic carcinoma and 15 cases of normal colonic mucosal tissue by avidin-biotin-peroxidase complex ( ABC)-immunohistochemistry. Results The level of histone H2B monoubiquitination was significantly reduced in poorly differentiated colonic carcinoma 19. 4% (6/31) compared with that of well-differentiated colonic carcinoma 49. 4%(42/85), moderately differentiated colonic carcinoma 49. 4% (42/85) and normal colonic mucosal tissue 86. 7% (13/15) (P0. 05). Conclusion Histone H2B monoubiquitination is obviously associated with the progression of primary colonic carcinoma. Although its specific mechanism still remains unclear, histone H2B monoubiquitination could be a novel potential molecular marker for early diagnosis, clinic treatment and prognosis evaluation.%目的：探讨组蛋白H2B单泛素化(histone H2B monoubiquitination, uH2B)在原发性结肠腺癌中的表达及其与临床病理参数的关系。方法采用免疫组化ABC法检测116例原发性结肠腺癌及15例正常结肠黏膜组织中uH2B的表达,并复习相关文献。结果 uH2B在低分化原发性结肠腺癌中的阳性率(19．4%,6/31)显著低于高+中分化腺癌(49．4%,42/85)及正常结肠黏膜组织(86.7%,13/15),差异有统计学意义(P0．05)。结论 uH2B作为一种抑癌因子可能与原发性结肠腺癌的恶化过程密切相关。其可能会成为原发性结肠腺癌早期检测、治疗及预后评估的新分子生物学标志并应用于临床,而其在结肠腺癌中的具体作用机制仍有待进一步研究。