超高效液相色谱-串联质谱快速测定食品中的新品种甜味剂爱德万甜

摘要

A new ultra-high performance liquid chromatography-tandem mass spectrometry with triple quadrupole method was developed,and validated for screening and confirmation of the new sweetener advantame in food.The sample was extracted with methanol-water(50∶50, v/v),followed by centrifugation.The supernatant was then filtered through a membrane filter.The determinant was performed on Agilent SB-C18(150 mm×2.1 mm,5 μm)column with gradient elution.The target compound was carried out by positive electrospray ionization (ESI﹢)under the multiple reaction monitoring(MRM)mode.While the qualitative screening was achieved with retention time and qualitative ion,the confirmation analysis was achieved with peak area and quantitative ion.The results showed that the limit of detection( LOD,S/N≥3)and the limit of quantification( LOQ,S/N≥10)were 0.03 and 0.10 mg/kg,respectively.The average spiked recoveries showed a variation from 80.3 to 98.0% for the three fortification levels.The results showed good linear relationships in the range of 0.01-1.0 mg/L of advan-tame with correlation coefficients above 0.997.This procedure was noticeably rapid,simple, sensitive,and accurate.Therefore,this method is suitable for batch determination of the new sweetener advantame in beverages,yogurt,and jelly.%建立了超高效液相色谱-串联三重四极杆质谱快速筛查和确证食品中的新品种甜味剂爱德万甜的方法.样品经甲醇-水(50∶50,v/v)超声提取,离心,取上清液过滤膜.采用 Agilent SB-C18(150 mm×2.1 mm,5 μm)色谱柱分离,梯度洗脱,在电喷雾离子源的正离子电离模式下,用多反应监测( MRM)方式采集数据,根据保留时间和定性离子对进行定性筛查,根据峰面积和定量离子进行定量分析.结果表明:方法检出限( LOD)为 0.03 mg/kg(信噪比 S/N≥3),定量限( LOQ)为0.10 mg/kg( S/N≥10),回收率为80.3%~98.0%,在0.01~1.0 mg/L范围内均有良好的线性,相关系数 r2均大于0.997.该方法快速、简便易行、灵敏、准确,适用于饮料、酸奶和果冻中的新品种甜味剂爱德万甜的批量检测.