采用液相沉积法在棉花纤维的表面成功沉积了纳米氧化锆颗粒,并将其装填在移液枪的吸头内,通过移液枪的抽吸实现对磷酸化多肽的萃取,萃取过程只需要2 min,方法简单、快速.该材料不仅可以从 β-酪蛋白酶解物这种简单的体系中萃取出9个磷酸化多肽,还可以从物质的量比为1:100的 β-酪蛋白酶解物和牛血清白蛋白(BSA)酶解物混合物这类含有大量非磷酸化多肽的复杂样品中萃取出4种磷酸化多肽,且没有非磷酸化多肽被检出,表现出较好的萃取选择性.将该材料应用于人血清和脱脂牛奶这两种复杂实际样品的酶解物中磷酸化多肽的快速富集萃取,分别检测出5种和9种磷酸化多肽,均表现出较好的选择性.%A kind of cotton-based fibrous adsorbent,zirconia nanoparticles coated cotton fiber (ZrO2-CF),was successfully prepared by liquid-phase deposition of zirconia nanoparticles on the surface of cotton fiber. And the ZrO 2-CF fibrous adsorbent was packed in pipet tips for the enrichment of phosphopeptide by pipetting up and down within 2 min,which was simple and fast. Nine phosphopeptides were identified from the digestion of β-casein after ZrO 2-CF extrac-tion. ZrO2-CF exhibited good selectivity in the enrichment of phosphopeptides from the diges-tion mixture of β-casein and BSA(molar ratio is 1:100)in which four phosphopeptides were identified. And it also showed excellent extraction efficiency in actual biological samples such as non-fat milk and human serum in which four and nine phosphopeptides were identified, respectively.
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