Institute of Materia Medica;
Chinese Academy of Medical Sciences;
Beijing;
Peking Union College Hospital;
Chinese Academy of Medical Sciences;
Beijing;
Peking Union College Hospital;
Chinese Academy of Medical Sciences;
Beijinguring autologous bone marrow graft in treatment of malignant diseases;
it is critical to purge malignant cells from the marrow. In the present study;
the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives;
YHpD;
10 μg/ml for 1 hr. and irradiated with blacklight (peak wavelength 395 nm;
light intensity 0.6 mW/cm2) for 5 minutes;
the survival rate of clonogenic cells decreased to <10%;
while that of bone marrow granulocyte macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (>80%). Similar results were obtained when human leukemic HL-60 cells were compared with human CFU-GM and mouse leukemic L615 cells with CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for Iselectively killing leukemic cells in bone marrow.;
progenitor; hematopoietic; granulocyte; minutes; macrophage; graft; photodynamic; counterpart; killing; autologous;