EXPRESSION OF IL-6, sgp130, IL-8 AND THEIR RECEPTORS INACUTE PROMYELOCYTIC LEUKEMIA DURING ALL-TRANSRETINOIC ACID INDUCTION TREATMENT

摘要

Objective: To evaluate the expression and itsclinical significance of interleukin 6 (IL-6), solubleglycoprotein 130 (sgp130), interleukin 8 (IL-8) and typeA interleukin 8 receptor (IL-8RA) in acutepromyelocytic leukemia (APL) patients during all-transretinoic acid (ATRA) induction treatment. Methods:Plasma and bone marrow mononuclear cell (MNC)culture supernatant IL-6, sgp130, IL-8 concentration of18 cases with APL were kinetically measured in vivo andin vitro (ELISA). Bone marrow MNC IL-8RA wasmeasured by now cytometry after being cultured withATRA (10-6mmol/L). Results: Plasma IL-6, sgp130, IL-8 levels were higher than normal (P<0.05), IL-6, sgp130levels correlated with white blood cell (WBC) counts(P<0.05) while IL-8 levels correlated with bodytemperature (P<0.05) at initial diagnosis. After 72-hourincubation with ATRA, concentration of IL-6 of bonemarrow MNC culture supernatant did not change, thato f sgp130 mildly decreased, and IL-8 significantlydecreased while the positive rate of IL-8RA on bonemarrow MNC increased. During ATRA treatment,plasma IL-6 changes were correlated with WBC counts.Peak levels of IL-6 and WBC were lower in patients whoreceived intermittent therapy than those who receivedcontinuous therapy. Plasma IL-6 and IL-8 wereincreased when complicated with infection and IL-8seemed more sensitive. Conclusion: Plasma IL-6, sgp130,IL-8 levels may reflect patients’ responsiveness to ATRAtreatment, and could be used to predicthyperleukocytosis and intercurrent infection. ATRAinduces APL cell differentiation possibly via sgp130signal transducer chain. Measurement of sgp130 hadcertain meaning to prognosis.