多孔硅Bragg反射镜适配子生物传感器检测心肌肌钙蛋白Ⅰ

摘要

Nano-porous silicon Bragg reflectors were fabricated using an electrochemical etching process. Then, the cTnI aptamers by binded with cTnI were immobilized to the porous silicon Bragg reflector surface using a crosslink method. After the binding between aptamers and the cTnI of different concentrations, the reflection spectrum red shifts of the porous silicon Bragg reflector were analyzed quantitatively. The result showed that the porous silicon Bragg reflector aptamer biosensor had high specificity. Furthermore,the procedures were label-free and detection time was reduced. The linear detection range of the sensor was 0. 05 -4 nmol/L, and the lowest detected concentration was 0. 05 nmol/L.%通过脉冲腐蚀法制备多孔硅Bragg反射镜,将心肌肌钙蛋白Ⅰ(cTnⅠ)适配子共价固定到多孔硅Bragg反射镜的孔洞中,发现适配子能与cTnⅠ分子特异性结合.定量分析不同浓度的cTnⅠ与适配子结合后多孔硅Bragg反射镜的反射谱峰位的红移情况.结果表明:基于多孔硅Bragg反射镜适配子生物传感器的光学检测具有良好的特异性,且具有免标记及检测时间短等优异性能.传感器的线性检测范围0.05～4 nmol/L,最低检测限为0.05 nmol/L.