Construction of Recombinant Plasmid Containing S.Mutans F-ATPase β Subunit Gene

摘要

objective: construct a homologous recombinant plasmid which was expected to be transformed into S.mutans Methods: a region at the 5’terminus of the S.mutans F-ATPase β subunit gene was amplified by PCR, the PCR product was inserted into vector pVA891,yielding recombinant plasmid.Results: the DNA sequence of the recombinant plasmid was identified correct in whole by restriction endonuclease and DNA sequence techniques.Conclusion: the recombinant plasmid of S.mutans DNA was cloned in effect,it may assist in construction of homologues recombinant mutant.