生防菌ANTI-8098A对青枯雷尔氏菌的抑菌特性

摘要

In this paper, the inhibition activity of Bacillus cereus strain ANTI-8098A at different culture stages against Ralstonia solanacearum, the influence of heat treatment on the inhibition activity and the absorbance values of the culture solution of strain ANTI-8098A at different wave length as well as the correlation analysis between the absorbance values and the inhibition activity were determined. The results showed that inhibition activity began at 12 h culture, getting highest at 48 h. When heated in boiling water bath, the EC_(50) of strain ANTI-8098A against R. solanacearum increased 14.40% . The absorbance values of the culture solutions at different culture stages at 190, 260, 280 or 400 nm were similar to each other. But during 450-900 nm, the absorbance values of the culture solutions increased with the passage of culture time. The trend of changes in the absorbance values was the same as the trend of changes in the inhibition effect. There was a negative correlation between the inhibition effect and the absorbance values of the culture solutions at 190 nm and positive at 450 nm. The results indicated the antibacterial substances produced by strain ANTI-8098A might not be proteins and could be marked at some wave length from 450 nm to 900 nm, especially at 450 nm.%本文测定了不同培养阶段的生防菌ANTI-8098A对青枯雷尔氏菌的抑制作用、加热处理对ANTI-8098A抑菌作用的影响、不同培养阶段的生防菌液在190～900nm波长范围内的光吸收值,分析了光吸收值与抑菌作用间的相关性.结果表明,生防菌ANTI-8098A的抑菌作用在培养12h后开始出现,48h时达到最强;生防菌液经沸水浴加热后会丧失小部分抑菌活性,使生防菌液对青枯雷尔氏菌的抑制中浓度(EC_(50))较未加热的提高14.40%;不同培养阶段的生防菌培养液在波长190、260、280和400nm处的吸收值相近,而在450～900nm的波长范围内,培养液的吸光值随着培养时间的延长而上升,这一变化趋势与培养液抑菌作用的变化趋势相一致;生防菌液的抑菌作用与其在190nm处的吸光值呈负相关,与其在450nm处的吸光值呈正相关.研究结果提示,生防菌ANTI-8098A的抑菌物质可能为非蛋白类的耐热毒素,并且能被450～900nm波长、尤其是450nm波长的光所标记.