乙烯雌酚对雌鼠生殖内分泌及下丘脑ERαmRNA表达的影响

摘要

Purpose To observe the effects of sex steroid hormones on reproductive endocrine and hypothalamic ERαmRNA expression in the female rats. Methods 16 rats from 32 female rats were randomly taken for the control group,and the remaining 16 rats were taken to couduct surgery and ovariectomy( tail clamp method was made), and after the success of modeling, it is the observation group. Each group was fed continuously for 4 weeks. Morphology of reproductive hormones and reverse transcription polymerase chain reaction (RT-PCR) were used to assay the hypothalamus,pituitary ERαmRNA expression. Results Compared with the normal control group,the wet weight index decreased significantly (P ＜ 0.01 ) in the observation group. At the same time there were significant changes in uterine morphology. The expression of hypothalamus,pituitary ERαmRNA in the observation group was significantly decreased compared with the control group (P ＜ 0.05). Conclusion The sex steroid hormones not only have an important physiological role on the reproductive system,and but also have an important role in the hypothalamus especially on the nervous system. The mechanism may be associated with elevated hypothalamic, pituitary ERαmRNA expression.%目的 观察性类固醇激素--乙烯雌酚对雌鼠生殖内分泌及下丘脑ERamRNA表达的影响.方法 健康大鼠32只随机取16只作正常对照组,其余16只均采取双侧卵巢切除手术加夹尾激怒法造模,造模成功后为观察组.两组分别连续灌胃生理盐水与乙烯雌酚4周,采用形态学观察生殖内分泌情况,采用逆转录聚合酶链反应(RT-PCR)法测定大鼠下丘脑ERαmRNA表达.结果 与正常对照组比较,观察组大鼠肾上腺湿重系明显降低(P<0.01).同时子宫形态也有明显变化.观察组大鼠下丘脑ERαmRNA的表达明显下降(P<0.05).结论 乙烯雌酚不仅对生殖系统有重要的生理作用,而且对神经系统尤其是下丘脑也具有重要作用.其作用机制可能与升高下丘脑ERamRNA的表达有关.