解毒活血法对UUO大鼠IL-1β和TNF-α的影响

摘要

目的:通过观察解毒活血法对大鼠肾组织白细胞介素-1β(interleukin-1β,IL-1β)、肿瘤坏死因子-α(tumour necrosis factor-α,TNF-α)的影响,探讨其对UUO大鼠的保护作用及可能机制.方法:采用结扎左侧输尿管的方法复制UUO大鼠模型.治疗组大鼠分别灌服缬沙坦26mg/kg·d,解毒活血中药12g/kg·d,24g/kg·d,共14d.放射免疫法检测IL-1β,免疫组化检测肾组织中TNF-α的蛋白表达.结果:模型组大鼠IL-1β和TNF-α的表达明显增高,各治疗组表达较模型组明显减弱.结论:解毒活血方药可降低肾组织中IL-1β和TNF-α的高表达,通过抑制炎症反应减缓肾小管间质纤维化的进程.%Aim: To observe the effects of Jiedu Huoxue therapy on the expression of interleukin-1β (IL-1β) and tumour necrosis factor-α( TNF-α) in UUO rats, and to explore the protective effect and possible mechanism of Jiedu Huoxue therapy in UUO rats. Methods: Rats were made into UUO model by ligaturing the left ureter and treated respectively with Valsartan 26mg/kg·d, Jiedu Huoxue decoction 12g/kg·d and 24g/kg·d for fourteen days. Take the left kidney to detect IL-1 βby radioimmunoassay. The protein expressions of TNF-α in rats ' kidney were detected by immunohistochemical. Results:The expression of IL-1β and TNF-α were increased significantly in the model group. After treatment, the expression of IL-1β and TNF-α were significantly down-regulated in the treatment groups. Conclusions Jiedu Huoxue decoction is able to reduce the high expressions of IL-1β and TNF-α, and to retard the progress of renal tubular interstitial fibrosis by inhibiting inflammatory reaction.