Objective To explore the changes in inositol requiring enzyme 1 (IRE1),apoptosis signal regulating kinase 1 (ASK1) and c-Jun N-terminal kinase (JNK) mRNA levels in peripheral blood CD4+ T cells of children with acute paraquat (PQ) poisoning.Methods Blood samples of 30 cases of acute PQ poisoning (PQ group),who visited Tianjin Children's Hospital from June 2014 to June 2016,with 18 male and 12 female,aged from 2 to 14 years old,were collected,and the clinical and laboratory data were documented.Peripheral venous blood samples were collected after paraquat was taken.Thirty healthy children at the same age and of the same sex were selected as a healthy control group,18 male and 12 female,aged from 2 to 14 years old.CD4+ T cells in the peripheral blood were separated,and IRE1,ASK1 and JNK mRNA levels in peripheral blood CD4+ T cells were measured by real time polymerase chain reaction (Real-time PCR) method.Specificity of PCR products was validated through agarose gel electrophoresis.The data were statistically analyzed by SPSS 13.0 software.Results All of the 30 children had mucosal lesions,nausea,vomiting and abdomen pain,19 cases with oliguria and anuria,16 cases with alimentary tract bleeding,12 cases with headache and dizziness,11 cases with short of breath,dyspnea and difficult breathing,8 cases with convulsion,5 cases with jaundice.The IRE1,ASK1 and JNK mRNA levels in PQ group were significantly higher than those in healthy control group (1.70 ± 0.16 vs.1.02 ± 0.18,3.56 ± 0.85 vs.1.05 ± 0.31,5.22 ± 0.87 vs.1.01 ± 0.33,t =15.26,15.21,24.78,all P ＜ 0.01).Conclusions PQ increased the expressions of IRE1,ASK1 and JNK in peripheral blood CD4+ T cells,which may be related to PQ-induced oxidative stress and immune activation and lead to a complex cytokine network via endoplasmic reticulum stress and CD4+ T cell apoptosis and then results in the occurrence and development of multiple organ failure.%目的 探讨急性百草枯(PQ)中毒患儿外周血CD4+T淋巴细胞肌醇依赖酶1(IRE1)、凋亡信号调节激酶1(ASK1)及c-Jun氨基末端激酶(JNK) mRNA水平的变化.方法 选择2014年6月至2016年6月于天津市儿童医院就诊的急性PQ中毒患儿30例,其中男18例,女12例;年龄2～ 14岁.记录患儿的一般临床资料及实验室检查资料,误服后2 ～20 h采集外周静脉血标本.选取同期来天津市儿童医院体检的健康儿童30例为健康对照组,男18例,女12例;年龄2～14岁.分离外周血CD4+T淋巴细胞,实时荧光定量聚合酶链反应(Real-time PCR)测定外周血CD4+T淋巴细胞IREt、ASK1及JNK mRNA的相对表达水平.PCR产物行琼脂糖凝胶电泳鉴定特异性.数据采用SPSS 13.0软件进行分析.结果 30例患儿均有口腔黏膜溃烂、恶心、呕吐、腹痛,少尿、无尿19例,消化道出血16例,头痛、头晕12例,胸闷、憋气、呼吸困难11例,抽搐8例,黄疸5例.PQ中毒组IRE1、ASK1及JNK mRNA相对表达水平均明显高于健康对照组(1.70 ±0.16比1.02 ±0.18、3.56±0.85比1.05 ±0.31、5.22 ±0.87比1.01 ±0.33,t =15.26、15.21、24.78,均P＜0.01).结论 PQ中毒患儿外周血CD4+T淋巴细胞IRE1、ASK1及JNK mRNA相对表达水平升高,可能与PQ所致氧化应激和免疫激活有关,通过内质网应激形成复杂的细胞因子网络,导致CD4+T淋巴细胞凋亡,影响多脏器衰竭的发生、发展.
展开▼
