毛细管电泳法手性拆分4种氨基酸和2种手性药物对映体

摘要

以双(6-氧-β-羧甲基-1,4-丁烯二酸单酯)-β-环糊精(DOCB-β-CD)作为手性添加剂,利用毛细管电泳对氨基酸和手性药物对映体进行拆分研究.以20 mmol/L磷酸盐为缓冲溶液,考察了手性添加剂的浓度及缓冲溶液的pH值与分离电压等对拆分效果的影响,并在其优化条件下,实现了4种DL-氨基酸(苯丙氨酸、色氨酸、酪氨酸和组氨酸)以及手性药物(罗格列酮和酮洛芬)对映体的基线分离.%Enantiomers of amino acids and chiral drugs were separated by capillary zone electrophoresis using double ( 6-O-β-carboxy-methyl-l, 4-maleic acid ester)-β-cyclodextrin ( DOCB-β-CD) as chiral selector. The buffer solution used was 20 mmol/L phosphate. The influences of DOCB-β-CD concentration, background buffer pH value, temperature and applied voltage were investigated. Under the respective optimum condition, enantiomers separation of four kinds of DL-amino acids ( Phenylalanine, Tryptophan, Tyrosine and Histidine) and two kinds of chiral drugs (rofecoxib and rosiglitazone) were attained with a clear separation from the baseline.