饲料粗纤维、中性洗涤纤维和酸性洗涤纤维残渣中各成分的研究

摘要

The present experiment was conducted to study the components in residues of crude fibers (CF),neutral detergent fiber (NDF) and acid detergent fiber (ADF) in feed.The contents of CF,NDF and ADF in rice bran,wheat bran,white clover,citron leaves,whole corn,whole wheat,Vicia villosa,rabbit feed,sorghum,oat straw,alfalfa,rice straw,triticale hay,Pennisetum hydridum,reed,peanut seedling,soybean stalk and rice husk powder were determined via polyester fiber mesh bag method,and determined the content and recovery of hemicellulose,cellulose,acid detergent lignin and acid insoluble ash in CF residue of feed,meanwhile,also determined the content of hemicellulose,cellulose,acid detergent lignin and acid insoluble ash in NDF and NDF residues of feed,to research the composition and the relationship between CF and ADF in CF,NDF and ADF residues.The results showed that the recovery of cellulose in CF residue was the highest (71.28％ to 105.03％).There was a highly positive correlation between ADF content (y1) and CF content (x1):y1=1.507 1+1.211 5x1(r=0.979 2,RSD=1.863 4,P＜0.01).There was a more correlation between the ADF without acid insoluble ash (Y2) content in ADF residue and CF content (x2) in ADF:y2=2.406 3+ 1.077 0x2(r=0.990 5,RSD=0.962 4,P＜0.01).We propose the accurate calculation method of hemicellulose and cellulose content:hemicellulose content =NDF content (without acid insoluble ash)-ADF content (without acid insoluble ash) and cellulose content=ADF content (without acid insoluble ash)-ADL content.%本试验旨在研究饲料粗纤维、中性洗涤纤维和酸性洗涤纤维残渣中各成分.试验采用聚酯纤维筛网袋法测定了米糠、小麦麸、白三叶、枸树叶、全株玉米、全株小麦、毛苕子、兔配合饲料、高粱、燕麦秸、苜蓿、稻秸、小黑麦干草、皇竹草、芦苇、花生秧、大豆秸和稻壳粉18种饲料的粗纤维(CF)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量,测定了饲料CF残渣中半纤维素、纤维素、酸性洗涤木质素和酸不溶灰分的含量及回收率,同时测定了NDF和ADF残渣中半纤维素、纤维素、酸性洗涤木质素和酸不溶灰分的含量,研究了饲料中的CF、NDF和ADF残渣中各成分含量及CF和ADF之间的相互关系.结果表明:所测18种饲料中的纤维素在CF残渣中的回收率最高(71.28％ ～ 105.03％);饲料ADF含量(y1)和CF含量(x1)之间呈高度正相关:y1 =1.507 1+1.211 5x1(r=0.979 2,RSD=1.863 4,P＜0.01);饲料ADF残渣中去除酸不溶灰分后的ADF含量(y2)和CF含量(x2)之间的相关性更高:)2=2.406 3+1.077 0x2(r=0.990 5,RSD=0.962 4,P＜0.01).由此提出饲料中半纤维素和纤维素含量的准确测定计算方法,即半纤维素含量等于NDF含量(无酸不溶灰分)减去ADF含量(无酸不溶灰分);纤维素含量等于ADF含量(无酸不溶灰分)减去酸性洗涤木质素含量.