The physical factors of the extracellular matrix have a profound influence on the differentiation behavior of mesenchymal stem cells(MSCs).In this study,different proportions of hydroxyapatite(HAp)were mixed into a poly(ethylene glycol)/silk fibroin(PEG/SF)solution to prepare scaffolds with different stiffness for studying the osteogenic effect on rat BMSCs in a biophysical microenvironment in vitro and in vivo.The amount of HAp ranged from 25 mg to 100 mg,which allowed the stiffness increasing from(80.98±17.45)kPa to(190.51±3.02)kPa be controlled suggesting the elastic modulus of the scaffolds increased with increasing HAp.
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