Positive charged silica nanoparticles, entrapping hydrophobic photosensitizer 2,9,16,23-tetraamino-phthalocyanine zinc (ZnPc(NH2 )4), have been synthesized in the nonpolar core of micelles by hydrolysis of triethoxyvinylsilane (TEVS) and 3-aminopropyltriethoxysilane (APTES). The water-soluble, stability, surface morphologies and charge were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and UV-visible spectrophotometer. The as-prepared nanoparticles are highly monodispersed spheres with uniform diameter (about 20 nm), and stable in aqueous system. Its average ζ potential value is 28. 8 ± 2. 79 mV and exhibits strong absorption peak at 714 nm. In addition, encapsulation of ZnPc(NH2)4 in the silica nanoparticles prevented ZnPc ( NH2 )4 from leaking and enhanced the anti-photobleaching. The ZnPc ( NH2 )4 entrapped-nanoparticles (SiP2@ZnPc(NH2 )4) can efficiently generat singlet oxygen as measured by chemical probe 1,3-diphenylisobenzofuran (DPBF). The toxicity of silica nanoparticles to cells has been investigated by the incubation of the ZnPc(NH2)4 entrapped-nanoparticles and the nanoparticles without ZnPc(NH2)4(SiO2-NH2) with living cancer cells (HeLa, U251, PC-12). The experimental result reveals that both SiO2@ZnPc(NH2 )4 and SiO2-NH2 particles have no significant toxicity when the concentration of the particles is below 300 mg/L.%通过在无极核微乳液中水解乙烯基三乙氧基硅烷(TEVS)和3-氨丙基三乙氧基硅烷(APTES),制备了疏水性光敏剂-2,9,16,23-四氨基酞菁锌负载的表面带有正电荷的二氧化硅纳米粒子(SiO2@ ZnPc( NH2)4).通过透射电镜(TEM)、Zetasizer Nano-ZS粒度仪(DLS)、紫外-可见分光光度计(UV- Vis)研究和表征了该纳米粒子的表面形貌、表面电荷、水溶性和稳定性.所合成的纳米粒子粒径约20 nm,颗粒为规则的球形,粒径较均一,具有很好的分散性,平均ζ电位值为(28.8±2.79 mV),在714 nm处有强吸收峰.此外,负载在纳米粒子中的ZnPc(NH2)4不易泄漏,增强了ZnPc(NH2)4的抗光漂白能力.以1,3-二苯基异苯并呋喃(DPBF)为探针分子,检测出SiO2@ ZnPc( NH2)4可以有效产生单线态氧.通过测量纳米粒子与活细胞(HeLa,U251,PC-12)孵育后的细胞活度来检测对细胞的毒性,结果显示,纳米粒子浓度≤300 mg/L时,未掺杂ZnPc( NH2)4的二氧化硅纳米粒子(SiO2-NH2)对细胞没有毒性；当其负载ZnPc( NH2)4后,对细胞没有显著毒性.
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