分子印迹胶体阵列检测对硝基苯酚

摘要

以对硝基苯酚(p-NP)为印迹模板,丙烯酰胺为功能单体,制备单分散的对硝基苯酚分子印迹胶体微球.通过垂直沉降法将分子印迹胶体微球自组装为分子印迹胶体阵列,采用胶带将分子印迹胶体阵列粘贴固定.固定于胶带上的分子印迹胶体阵列膜显示出良好的稳定性,而且对目标分子p-NP具有明显的光学响应.分子印迹微球吸附目标分子发生溶胀,引起胶体阵列溶涨,分子印迹胶体阵列( MICA)反射峰位置发生移动.实验结果显示,MICA随着p-NP浓度增加,反射峰红移近60 nm,MICA表面颜色由红色逐渐变为蓝紫色；而非印迹胶体阵列红移量只约40 nm.MICA简化了光子晶体凝胶传感材料的制备步骤,为开发新型高性能生化传感器材料提供了新思路.%p-Nitrophenol molecularly imprinted colloidal microspheres were prepared by polymerization of functional monomers (acrylamide, AM) and template ( p-nitrophenol, p-NP) in poly (methyl methacrylate, PMMA) pre-polymerization solution. Molecular imprinted colloidal array (MICA) was prepared using the molecularly imprinted colloidal microspheres by self-assembly and then fixed by an adhesive tape. The MICA on the adhesive tape is easy to handle and has strong stability. This material senses P-nitrophenol in aqueous solution by shifting reflection wavelength. The adsorption of p-nitrophenol into molecularly imprinted colloidal microspheres would swell the microspheres, thus leading to a reflection peak red shift. The reflection peak red shifted nearly 60 nm when the concentration of p-NP increases from 0 mmol/L to 30 mmol/L, while non-imprinted molecularly imprinted colloidal array (NICA) red shifted about 40 nm. The color of MICA changed from red to blue-violet. This method simplifies the preparation of photonic crystal material which is an efficient substance for developing high performance chemical/biological sensors.