Photolithography was used to pattern hydrophobic design on common quantitative filter paper, based on which we successfully fabricated the paper-based micro-zone plates. In order to improve its protein-absorbing capability for further signal magnification, modified silicon dioxide (SiO2 ) micro-beads were deposited onto the surface of paper. Then indirect-ELISA for goat anti-rabbit IgG was applied on the 36-well plates. Taking enzyme linked goat anti-rabbit IgG as an example, loading SiO2 beads could remarkably increase the quantity of the absorbed protein by 20% -700% . When this method was used for goat anti-rabbit IgG detection, signals were magnified by 20% -150% , and the linear range of detection was from 3×l0-12 mmol/zone to 3×10-8 mmol/zone under given conditions. In this manner, bio-reagent consumption was reduced to 3-5 ΜL per zone, test time dropped to 25min per plate and the cost of fabrication reduced to $1.1 per plate, which indicated a promising application foreground for new disease diagnosis devices.%用光刻法在普通定量滤纸上制作出了疏水图案,得到了滤纸微孔板.将表面修饰后的SiO2微球负载在滤纸表面,以提高滤纸纤维吸附蛋白质的能力,基于此建立了以间接酶联免疫吸附法测定羊抗兔免疫球蛋白的方法.以酶标兔抗羊IgG为例,负载SiO2微球后,滤纸微孔板吸附该蛋白质的量提高了20％ ～ 700％；将SiO2负载法应用于羊抗兔IgG的检测时,可使信号增强20％～150％.在本实验条件下,测定羊抗兔IgG的线性范围为3×10-12 ～ 3×10-8 mmol/孔.本方法使生物免疫试剂消耗量减少至3～5μL/孔、检测时间降至20min/板、滤纸微孔板制作成本可低至约每板1.1美元,为研制准确、快速和低成本的疾病检测设备提供了新的思路和可能性.
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