马铃薯‘H-027’与‘陇薯6号’和‘陇薯7号’ 杂种优良单株的SSR分析

摘要

In order to determine the authenticity of F1 superior individuals of potato hybrid, the SSR molecular marker technology was used to identify the F1 superior individuals of two hybrid combinations ‘H-027’ בlongshu No.6’ and ‘H-027’ בlongshu No.7’. Three suitable primer pairs S7, S170 and S174 were selected from 87 SSR primer pairs. Using the SSR primers S7 and S170, a total of 25 true hybrid plants were identified from 30 superior individuals of the hybrid combination ‘H-027’× ‘longshu 6’. Using the primers S170 and S174, all the 14 superior individuals of the hybrid combination ‘H-027’ בlongshu 7’were identified as true hybrid plants.%为了明确马铃薯杂种F1单株的真实性，利用SSR分子标记技术对‘H-027’ב陇薯6号’和‘H-027’ב陇薯7号’这2个马铃薯杂交组合F1的优良单株进行鉴定。试验从87对SSR引物中筛选出3对适宜引物S7、S170和S174；用引物S7和S170从马铃薯‘H-027’ב陇薯6号’的杂种F1的30个优良单株鉴定出25个真实杂交种单株；用引物S170和S174鉴定出‘H-027’ב陇薯7号’杂种F1的14个优良单株均为真实杂交种。