南荻遗传转化系统的建立及转基因植株的获得

摘要

The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflorus were established at very high frequency. Potato proteinase inhibitor II (pinII) gene were introduced into the callus of Miscanthus sacchariflorus and some transgenic plants were obtained by the particle bombardment transformation system. PCR and Southern analysis showed that target genes were integrated into the genome of these transgenic plants.%建立了南荻愈伤组织高频诱导与再生系统及其转化愈伤组织的筛选系统,并通过本研究所建立的基因枪转化系统,将马铃薯蛋白酶抑制基因导入南荻愈伤组织而获得转基因植株。