Objective To investigate the clinical significance of interleukin - 13 ( IL - 13 ) and the application value of induced sputum technique in the diagnosis of airway inflammation in asthmatic patients. Methods Thirty - four patients with asthma ( 12 patients at mild and moderate attack stages, 12 at sever attack stage, and 10 at remission stage ) as well as 10 healthy individuals were enrolled in this study. The levels of IL - 13 in induced sputum and serum were determined by enzyme linked immunosorbent assay ( ELISA ), and their association with the leukocyte levels and pulmonary function were explored. Results The levels of IL - 13 in the induced sputum and serum in these four groups showed significant difference ( P ＜0. 05 ) . The levels of IL - 13 in induced sputum and serum were positively correlated with the ratios of eosinophils/leukocytes ( P＜0. 05 ), and negatively correlated with forced expiratory volume ( FEV ), FEV1/FVC , and peak expiratory flow ( PEF ) ( P ＜0. 001 ) . There was significant correlation ( P = 0. 021 ) between the ratios of eosinophils/leukocytes in the induced sputum and in the serum in severe attack group. The ratios of eosinophils/leukocytes of the induced sputum showed no significant defference between the patients at mild/moderate attack stage and those at remission stage ( P ＞ 0. 05 ) . Conclusion It was safe and effective to detect airway inflammation using induced sputum technique. The determination of IL - 13 levels in the induced sputum and serum is useful for the diagnosis and prognosis of asthma.%目的 探讨白介素13(IL-13)在支气管哮喘发病中的作用以及诱导痰IL-13在哮喘气道炎症中的应用价值.方法 采用酶联免疫吸附实验检测支气管哮喘重度发作(重度组)12例、轻～中度发作(轻～中度组)12例、缓解组10例以及10例健康者(对照组)的诱导痰及血清中IL-13水平,并分别与痰液、血液中白细胞分类以及肺功能指标进行相关性分析.结果 支气管哮喘患者IL-13水平在重度组、轻～中度组、缓解组、对照组的诱导痰和血清中的差异均有统计学意义(P＜0.05);并且分别与痰液和血液中嗜酸粒细胞分数呈正相关(P＜0.05),与肺功能指标呈负相关(P＜0.001).重度发作组痰液和血液的嗜酸粒细胞分数呈正相关(P=0.021).诱导痰嗜酸粒细胞分数在缓解组和轻～中度组中无直线相关(P＞0.05).结论 利用诱导痰检测支气管哮喘患者的气道炎症是一种安全、有效的方法.支气管哮喘患者诱导痰及血清IL-13水平有助于诊断哮喘和判断病情.
展开▼
