Taqman PCR-based Methods for Rapid Detection o＇ Salmonella in Pet Food

摘要

[Objective] To establish a Taqman real-time PCR for detection of Salmonella in pet food. [Method] A pair of primers and a probe were designed based on published nucleotide sequence of invA gene encoding the invasion protein of Salmonella enterica. [Result] The assay detects Salmonella specifically. The detection limit of the real-time PCR was 17 CFU/test (25 μL/test) for the positive strain. This method was effective to detect artificially contaminated pet food. [Conclusion] The results showed that Taqman PCR assay was rapid and accurate for detection of Salmonella from infected pet food.