Objective: To explore the expression and clinical significance of serum long chain non-encoded RNAANRIL (LncRNA ANRIL) in chronic heart failure (CHF) patients. Methods: Our research included in 2 groups: CHF group, n=120 patients treated in our hospital and Control group, n=28 healthy subjects at the same period of time. Based on NYHA classification, CHF patients were further divided into 4 subgroups: NYHAⅠ subgroup, n=28, NYHA Ⅱ subgroup, n=34, NYHA Ⅲ subgroup, n=35 and NYHA Ⅳ subgroup, n=23. Expressions of serum ANRIL and cystatin C were examined by real time fluorescence quantitative PCR (QRT-PCR) and compared between 2 groups; the relationship between ANRIL, cystatin C and cardiac function were studied. Results: Compared with Control group, CHF group had increased serum levels of ANRIL and cystatin C, P<0.05. ANRIL expression was gradually increasing by cardiac function decreasing in CHF I to CHF Ⅳ subgroups, P<0.05. Correlation analysis found that serum level of ANRIL was positively related to cystatin C (r=0.873, P<0.001). ANRIL was elevated by increased left ventricular end diastolic diameter and decreased ejection fraction, both P<0.05. Conclusion: Serum ANRIL level has been related to CHF at certain degree which may indirectly reflect the severity of HF in relevant patients.%目的:探讨检测长链非编码位于细胞周期激酶抑制因子4(INK4)基因座中反义非编码RNA(LncRNA ANRIL)在慢性心力衰竭(CHF)患者血清中的表达及临床意义.方法:选择我院收治的冠心病CHF患者(CHF组)120例,按美国纽约心脏协会(NYHA)评价心功能,即Ⅰ级28例、Ⅱ级34例、Ⅲ级35例、Ⅳ级23例;收集同期健康体检者28例作为对照组,采用实时荧光定量聚合酶链式反应检测两组血清LncRNA ANRIL及胱抑素C的表达,分析血清LncRNA ANRIL的表达与胱抑素及左心室射血分数的相关性.结果:与对照组比较,CHF患者血清LncRNA ANRIL及胱抑素C的表达水平明显增高,差异有统计学意义(P<0.05).心功能Ⅰ级~Ⅳ级的CHF患者中,血清LncRNA ANRIL的表达水平随心功能的降低而逐渐升高,各组间差异具有统计学意义(P<0.05).相关分析发现血清LncRNA ANRIL与胱抑素C的表达呈正相关关系(r=0.873,P<0.001).血清LncRNA ANRIL水平随着左心室舒张末期内径的增大而升高,随着左心室射血分数的降低而升高,两者之间差异均有统计学意义(P均<0.05).结论:血清LncRNA ANRIL水平的高低与CHF具有一定的相关性,可以间接的反映心力衰竭的严重程度.
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