大白菜游离小孢子培养技术高效体系的研究

摘要

Taking Chinese cabbage〔Brassica campestris L. ssp. pekinensis(Lour)Olsson〕‘Jihong 82’,some of #534DH and #438 population as testing material,studies the effect of mechanical extraction and artificial extrusion on micro-spore viability and extraction quantity.The result indicates that mechanical extraction can replace the artificial one,get multiple samples during short period of time,and rapidly adjust the concentration of microspores culture.40 suitable buds are collected in 5 mL centrilfuge tubes,3 000 r·min-1 micro-spores gained by 10 s smashing extraction are isolated in 25 mL NLN-13 medium.Thus,micro-spore concentration is insured in suitable culture scope,and successfully induced to embryogenesis.%以大白菜吉红82、#534DH群体及#438群体为试材，研究了利用细胞破碎仪机械提取小孢子与人工挤压提取小孢子对大白菜游离小孢子活力及提取量的影响。结果表明：机械提取替代人工挤压可在短时间内同时提取多份样品，并可快速调整小孢子的培养浓度。收集40个适期花蕾于5mL离心管内，3000r·min-1破碎10s，将提取的小孢子定容于25mLNLN-13培养基，可确保小孢子浓度在适宜培养范围内，并成功诱导胚胎发生。